三色堇与角堇种间遗传连锁图谱构建
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引用本文:王 虎,刘佳燚,董丽婷,崔亚会,杜晓华.三色堇与角堇种间遗传连锁图谱构建[J].西北植物学报,2021,41(3):398~405
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作者单位
王 虎,刘佳燚,董丽婷,崔亚会,杜晓华* (河南科技学院 园艺园林学院河南新乡 453003) 
基金项目:河南省大学生创新项目(S202010467010)
中文摘要:该研究以二倍体三色堇和角堇为亲本杂交产生的66株F2代分离群体为作图群体,采用SRAP标记技术进行基因分型,利用JoinMap4.0软件构建了首张三色堇与角堇的种间遗传连锁图谱。结果表明:(1)从256对SRAP引物组合中筛选获得50对多态性好、标记位点清晰且稳定的引物组合。(2)通过对三色堇F2代群体的PCR扩增,共获得118个SRAP多态性标记位点,其中偏分离标记率为24.6%,符合遗传作图需要。(3)成功构建了三色堇和角堇的种间分子遗传连锁图谱,该图谱有15个连锁群,67个SRAP标记,连锁群长度范围1.6 ~ 52.2 cM,覆盖基因组总长度327.9 cM,标记间平均图距为4.9 cM。研究结果为三色堇和角堇高密度遗传图谱构建和重要性状的基因定位及分子标记辅助选择育种奠定了基础。
中文关键词:三色堇  角堇  SRAP  遗传图谱
 
Construction of Genetic Linkage Map for Interspecies of Viola tricolor and V. cornuta
Abstract:In this research, the first interspecific genetic linkage map of Viola tricolor and V. cornuta was constructed with 66 individuals of F2 population crossed between V. tricolor and V. cornuta using SRAP marker technique for genotyping. The results revealed that: (1) fifty SRAP primer pairs with more polymorphic, clear and stable bands were selected from 256 primer combinations. (2) A total of 118 SRAP polymorphism markers were obtained after PCR amplification of the F2 segregation population with 24.6% distorted marker, which met the requirements for genetic map construction. (3) The interspecific genetic map of V. tricolor and V. cornuta was successfully constructed, consisting of 15 linkage group with 67 SRAP markers, and covering the genome of 327.9 cM. The length of linkage groups ranged from 1.6 cM to 52.2 cM, and the average distance between markers is 4.9 cM. This study laid the foundation for high resolution map construction, gene mapping and molecular marker assisted selection in V. tricolor and V. cornuta breeding.
keywords:Viola tricolor  V. cornuta  SRAP  genetic map
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