219份枣种质资源果实三萜酸含量分析
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引用本文:薛晓芳,赵爱玲,王永康,任海燕,李登科,李 毅.219份枣种质资源果实三萜酸含量分析[J].西北植物学报,2021,41(3):480~492
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作者单位
薛晓芳,赵爱玲,王永康,任海燕,李登科*,李 毅 (山西农业大学 果树研究所,果树种质创制和利用山西省重点实验室太原 030031) 
基金项目:国家重点研发计划(2018YFD1000607);
中文摘要:以219份枣种质脆熟期果实为试材,采用超高效液相色谱(UPLC)法检测不同枣种质果实中的山楂酸、桦木酸、齐墩果酸和熊果酸4种三萜酸组分含量,并进行描述统计分析、相关性分析和聚类分析,进而筛选三萜酸含量较高的优特异种质。结果表明:(1)枣果中总三萜酸(TTA)含量的变化范围95.72 ~ 737.82 μg·g-1,平均值306.83 μg·g-1,变异系数为29.02%,含量最高的为‘大荔小圆枣’。4种组分以山楂酸含量最高,其次为桦木酸,齐墩果酸和熊果酸含量较低,各组分在不同枣种质中都存在丰富的遗传多样性,其中熊果酸变异系数最高(60.75%)。(2)正态性检验结果表明,各种质的山楂酸、桦木酸和总三萜酸含量服从正态分布,齐墩果酸和熊果酸含量不服从正态分布。(3)相关性分析结果显示,总三萜酸含量与4个组分均呈极显著正相关关系,组分间也存在相关性,山楂酸含量与桦木酸呈极显著正相关关系,桦木酸含量与齐墩果酸呈极显著正相关关系,齐墩果酸含量与熊果酸呈极显著正相关关系。(4)聚类分析将219份枣种质划分为4大类群,分别为熊果酸含量最高的类群,齐墩果酸和熊果酸含量较高类群,各组分中低含量类群以及山楂酸、桦木酸和总三萜酸含量较高类群。(5)筛选出13份各组分或总三萜酸含量较高的优特异种质,为枣三萜酸相关功能产品的开发提供原料选择。
中文关键词:  种质资源  果实  三萜酸组分
 
Analysis of Triterpenic Acid Contents in Fruits of 219 Jujube Germplasm Resources
Abstract:This study aims to make clear the characteristics of triterpenic acid components and contents in fruits of jujube germplasm resources and to provide theoretical reference for the development and utilization of triterpenic acid. The crisp mature fruits of 219 jujube germplasm resources were used as the test materials, by using Ultra High Performance Liquid Chromatography (UPLC) method, the triterpenic acid components including maslinic acid, betulinic acid, oleanolic acid and ursolic acid were determined, and we performed the descriptive statistics, correlation and cluster analysis to screen the excellent resources of higher triterpenic acid contents. The results showed that: (1) the distribution range of total triterpenic acid (TTA) in jujube resources was 95.72-737.82 μg·g-1, and the average content was 306.83 μg·g-1 with variation coefficient 29.02%, the highest content resource was ‘Dalixiaoyuanzao’. In terms of the four components, the contents of maslinic acid were the highest, followed by betulinic acid, the contents of oleanolic acid and ursolic acid were lower. The genetic diversity of each component was abundant in different jujube resources, among which the variation coefficient of ursolic acid was the highest (60.75%). (2) The normal test results showed that the contents of maslinic acid, betulinic acid and total triterpenic acid were normally distributed, and the contents of oleanolic acid and ursolic acid were not normally distributed. (3) The correlation analysis results showed that the total triterpenic acid contents were significantly positive correlated with all four components, and there were also correlations between components, the maslinic acid contents were significantly positive correlated with betulinic acid, the betulinic acid content were significantly positive correlated with oleanolic acid, and the oleanolic acid content were significantly positive correlated with ursolic acid. (4) Cluster analysis can divide 219 resources into 4 groups, which were the groups with the highest content of ursolic acid, the groups with higher contents of oleanolic acid and ursolic acid, the groups with lower content of each components, and the groups with the higher contents of maslinic acid, betulinic acid and total triterpenic acid. (5) We screened out 13 germplasm resources including higher contents of each component and total triterpenic acid, which can provide raw materials for the development of triterpenic acid related functional products.
keywords:Chinese jujube  germplasm resources  fruits  triterpenic acid components
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