TCP plays an important role in abiotic stress such as high salt and drought. The putative HrTCP were explored and identified from seabuckthorn transcriptome data and analyze their structure and bioinformation. The key genes involved in abiotic stress were obtained by homologous sequence alignment and qRTPCR., we further analyzed the gene structure and function of its corresponding transcription factors of seabuckthorn under abiotic stress. The results showed that: (1) 11 putative seabuckthorn HrTCPs were confirmed and named HrTCP2/4/7/8/11/13/15/17/18/19/20. The number of amino acids encoded by 11 HrTCPs was 218-590, the relative molecular weight of the proteins was 23.44-61.78 kD, the isoelectric point range of the proteins was 6.09-9.72, and the subcellular localization of the proteins was in the nucleus except for the subcellular localization of HrTCP13/17/18. (2) qRTPCR showed that the genes of HrTCP can response to abiotic stress, but the response degree of different members is different under stress treatments. The expression levels of HrTCP4/7/19/20 in 15% PEG6000 and 200 mmol/L NaCl were significantly upregulated and among which the expression level of HrTCP20 was the most significantly higher compared with the control, which was 24 and 23 times of the control. (3) After the ABA (0.1 mmol/L) and MeJA(0.1 mmol/L) treatments, HrTCP7/19/20 genes also showed an upregulated expression trend, especially the expression level of HrTCP19 was most significantly increased under the ABA treatment, which was 16 times of the control. The expression level of HrTCP20 was most significantly upregulated in response to MeJA treatment, which was 5 times of the control. Our present study indicated that the members of the HrTCP transcription factor family can be expressed by drought, salt, and hormones to response drought stress of seabuckthorn.