‘砀山酥梨’黄化叶复绿过程中内源IAA含量及其信号转导基因的表达量分析
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国家现代农业产业技术体系建设专项(CARS2914);


Analysis of Endogenous IAA Content and Signaling Genes Expression in Retrieved Leaves of ‘Dangshansuli’ Pear (Pyrus bretschneideri Rehd.)
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    摘要:

    为探讨IAA在梨黄化叶复绿过程中的作用,以‘砀山酥梨’(Pyrus bretschneideri Rehd.)黄化植株与正常植株为试材,对生长期黄化植株叶面喷施0.2% FeSO4溶液,于处理后第3、6、9、12天分别观察黄化叶复绿情况,取样测定黄化叶片复绿过程中各时期叶片与对照组(正常、黄化)叶片的Fe2+含量、内源IAA含量、IAA相关信号转导基因相对表达量,并分析Fe2+、IAA含量及相关基因表达量之间相关性。结果表明:(1)FeSO4溶液处理可使梨黄化叶片复绿,且黄化叶面在处理后第3天开始出现复绿斑点,在处理后第6、9天时出现大范围的复绿斑块,第12 天整叶复绿。(2)ELISA测定结果显示,对照组正常叶的内源IAA含量显著高于对照组黄化叶,FeSO4处理后各个时期的复绿叶内IAA含量均显著高于对照组(正常叶和黄化叶)。(3)qRTPCR结果显示,对照组黄化叶内AUX1.1/1.3、GH3.1/3.2/3.5、SAUR1/2/3/4、AUX/IAA1/2/3/7表达量均显著高于对照组正常叶,FeSO4处理后其表达量大多较黄化叶显著减少;而对照组黄化叶内TIR1.1、GH3.3/3.4、AUX/IAA4/5/6表达量均显著低于对照组正常叶,FeSO4处理后其各时期表达量均较黄化叶显著增加。(4)对照组黄化叶内ARF3/4/5/6/7/8/9/10/15/17/18/19/20/21/22/23表达量均显著低于正常叶,FeSO4处理显著促进了ARF3/5/6/9/19/20/21/22/23的表达;而对照组黄化叶内ARF1/2/11/12/13/14表达量显著高于正常叶,FeSO4处理则显著抑制了ARF1/2的表达。(5)相关性分析结果表明,FeSO4处理后复绿叶内显著增加的Fe2+含量主要与AUX/IAA5、AUX/IAA6表达量显著上调相关;而IAA含量的显著增加则与GH3.3表达量显著上调、与SAUR1/2表达量显著下调相关。两者之间相关网络图错综复杂,其简要途径为:Fe2+AUX/IAA5、AUX/IAA6→(ARF14)→ARF5→SAUR1、AUX/IAA1→SAUR2/TIR1.1/GH3.3→IAA。研究认为,IAA及其信号转导基因在FeSO4所诱导的‘砀山酥梨’缺铁黄化叶复绿过程中可能发挥重要作用。

    Abstract:

    To reveal the role of IAA and its signaling genes in the retrieved green process of pear leaves, we took chlorotic and normal plants of ‘Dangshansuli’ (Pyrus bretschneideri Rehd.) as test material. 0.2% FeSO4 solution was sprayed on the chlorotic leaves and distilled water was used for both normal and chlorotic leaves as control during the growing season. Then we observed the leaves phenotypic changes on the 3rd, 6th, 9th, and 12th day after FeSO4 application, respectively. We sampled normal, chlorotic leaves in control (CN and CC) and retrieved green leaves on the 3rd, 6th, 9th, 12th day after FeSO4 application, to determine the Fe2+ content, endogenous IAA content and IAA signaling genes expression. Furthermore, relevance analysis was performed among those indexes. The results were shown as follows: (1) application FeSO4 could convert chlorotic leaves to green, green spots appeared on the chlorotic leaves surface on 3rd day, and green plaque appeared on the both 6th and 9th day after spraying FeSO4. The whole leaves presented green on the 12th day. (2) The endogenous IAA content in CN was significantly higher than that in CC, and after spraying FeSO4, it obviously increased in retrieved green leaves compared with both CN and CC in all periods. (3) AUX1.1/1.3, GH3.1/3.2/3.5, SAUR1/2/3/4 and AUX/IAA1/2/3/7 expression in CC were prominently higher than that in CN, and most of them were notably inhibited in all period after spraying FeSO4. In contrast, TIR1.1, GH3.3/3.4 and AUX/IAA4/5/6 expression in Cc were distinctively lower than that in CN, and they were all apparently promoted by FeSO4 application. (4) ARF3/4/5/6/7/8/9/10/15/17/18/19/20/21/22/23 in Cc were clearly decreased compared with CN, and FeSO4 application significantly promoted the expression of ARF3/5/6/9/19/20/21/22/23; on the contrary, ARF1/2/11/12/13/14 were remarkably increased in Cc compared to CN, and FeSO4 application remarkably suppressed the expression of ARF1/2. (5) The relevance analysis showed that remarkably increased Fe2+ content in retrieved green leaves was positively correlated to the upregulation of AUX/IAA5 and AUX/IAA6, while IAA content was positively correlated with the increased GH3.3 expression and negatively correlated with the decreased SAUR1/2 expression. The network diagram of the association between the two above is complex, which can be simplified into Fe2+AUX/IAA5, AUX/IAA6→(ARF14)→ARF5SAUR1, AUX/IAA1→SAUR2, TIR1.1, GH3.3→IAA. The study suggested that IAA and its signaling pathway might play a crucial role in the retrieved green process induced by spraying FeSO4 on irondeficiency chlorotic leaves of ‘Dangshansuli’ pear.

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贾 兵,郭国凌,余 桃,等.‘砀山酥梨’黄化叶复绿过程中内源IAA含量及其信号转导基因的表达量分析[J].西北植物学报,2021,41(4):595-605

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  • 在线发布日期: 2021-05-21
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