新疆3种甘草根际土壤丛枝菌根真菌群落的多样性分析
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国家自然科学基金(41561010,31560177)


Comparative Study of Rhizospheric Arbuscular Mycorrhizal Fungi from Three Medicinal Licorice Plants of Xinjiang
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    摘要:

    为探究新疆地区药用甘草根际土壤丛枝菌根真菌的群落结构受宿主植物种类、土壤深度和土壤理化性质的影响,该实验采集了新疆地区乌拉尔甘草、胀果甘草、光果甘草根际 0~20 cm、 20~40 cm、 40~60 cm 3个土层的土壤样品,基于 Illumina Miseq 高通量测序平台测定 AM 真菌群落结构和多样性,结合土壤理化性质,分析新疆地区药用甘草根际土壤丛枝菌根真菌与土壤因子之间的关系,以明确丛枝菌根真菌在提高栽培甘草的有效成分含量等方面的作用。结果表明:(1)实验共分离到 1 门 1 纲 5 目 5 科 5 属 34 种 AM 真菌,球囊菌门(Glomeromycota)为绝对优势门,优势属为球囊霉属(Glomus)和类球囊霉属(Paraglomus)。(2)类球囊霉属内的各种之间呈正相关关系,球囊霉属内的各种之间也多呈正相关关系,而类球囊霉属与球囊霉属属间各种多呈负相关关系。(3)主坐标 PCOA分析显示,3种甘草之间的 AM 真菌群落完全分开,同种甘草在不同土壤深度之间有部分重叠;相关性热图显示,球囊霉属与土壤总磷呈极显著正相关关系(P<0.01),与土壤总钾(P<0.001)、土壤有机质含量(P<0.01)均呈极显著负相关关系;类球囊霉属与土壤总磷(P<0.05) 呈显著负相关关系,与土壤总钾(P<0.001)、土壤总盐(P<0.01)、土壤有机质含量(P<0.01) 均呈极显著正相关关系。研究认为,3种甘草的种间差异较为明显,2个优势属的属内处于互利共生关系,而属间处于竞争关系;土壤有机质对2个优势属具有一定影响;类球囊霉对盐的耐受性较强且多发现于胀果甘草土壤中,胀果甘草的耐盐碱能力强可能与其有关。

    Abstract:

    To explore the rhizospheric AMF community structure and diversity of three licorice varieties, as well as their relationship with the host plant, soil depth, physical and chemical properties, we collected the soil from a depth of 0-20 cm, 20-40 cm, 40-60 cm from licorice plants root rhizosphere of Xinjiang region, and employed the Illumina Miseq highthroughput sequencing platform to investigate the structure and diversity of these AM fungal communities, combined with the soil physical and chemical properties. (1) In this study, a total of 1 phyla, 1 class, 5 orders, 5 families, 5 genera, 34 species of AM fungi were isolated, Glomeromycota emerged as the dominant phyla, Glomus and Paraglomus emerged as the dominant genera as per the bioinformatics analysis. (2) Paraglomus is positively correlated, and Glomus is also positively correlated, while Paraglomus is general negatively correlated with Glomus. (3) The PCOA analysis showed that the AM fugal communities of the three licorice were completely separated, the same licorice partially overlapped in different soil depths. The genus Glomus was found to be positively correlated to total phosphorus (P<0.01), and negatively correlated to total potassium (P<0.001) and Organic of soil content (P<0.01). The genus Paraglomus was found to be negatively correlated to the total phosphorus of soil (P<0.001), and significantly positively correlated to total potassium (P<0.001), soil total salt (P<0.01), and soil organic content (P<0.01). The differences among the three species of licorice were obvious. The two dominant genera were in mutualism within the genus, but in competition among the genera. soil organic content had a certain influence on the two dominant genera. The tolerance of Paraglomus to salt is stronger and mostly found in the soil of Glycyrrhiza inflata Bat., G. inflata has strong salttolerant ability may be related to it.

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高甜甜,王仲科,贺亚玲,等.新疆3种甘草根际土壤丛枝菌根真菌群落的多样性分析[J].西北植物学报,2021,41(4):643-653

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  • 在线发布日期: 2021-05-21
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