With the natural forest in Mulinzi National Nature Reserve in Southwest, Hubei Province as the research object, we took the trees with DBH (diameter at breast height) ≥ 5 cm in forests as specific analysis objects. By analyzing the important value of tree species and their Uniform angle index, Dominance, Mingling stand spatial structure parameters, such as a distribution, stand spatial distribution pattern, size, degree of differentiation, trees mixed degree and the change, and through the stand spatial structure analysis of bivariate distribution, we revealed the spatial structure characteristics and dynamic changes of the stand in five years, to provide accurate data for the sustainable management of the forest type of this stand, in order to promote the ideal stand structure and maintain the species diversity in this area. The results showed that: (1) the main edificator in the 2014 and 2019 standing forest were Cyclobalanopsis glauca, which was dominant in the forest, and the important value of Carpinus turczaninowii increased by 0.63%, and its relative importance in the standing forest ranked second after Cunninghamia lanceolata. It indicated that C. turczaninowii had a stable population structure in this forest; (2) The standing fores were still in a slightly conglomerate distribution state as a whole, and the strength of each tree species was mixed. The average mixing degree increased by 0.02, and the average size ratio decreased by 0.01. The growth of standing forest was still in a moderate state, the overall spatial structure of the standing forest changed little in the past 5 years, but it was in the transition stage from the current standing forest structure to the ideal structure; (3) In the binary distribution of standing forest spatial structure of each group with pairwise combination of structural parameters, the change trend of different value grades of each parameter was basically unchanged, but the binary distribution results of Dominance and Mingling degree show that individual forest trees under situation of absolute disadvantage, disadvantage and zerodegree mixing, and weakdegree mixing were decreased. Meanwhile, the stability of standing forest was improved. Studies have shown that, although the dynamic change of spatial structure of natural stands in the study area is not obvious, they are developing toward better and more stable structure.