The Arabidopsis ABI5 subfamily transcription factors AtDPBFs (DC3 promoter binding factors)/ABFs (ABRE binding factors) are composed of 9 highly homologous members, which are involved in seed maturation and dormancy, flowering time, root growth, ABA signal transduction and stress responses. This study used the qRTPCR method to analyze the relative mRNA content changes of 9 genes of ABI5 subfamily in different tissues and growth stages, and the response to ABA, high salt, hypertonicity and low temperature in seedling stages in order to clarify the similarities and differences of the biological functions of the nine genes in Arabidopsis. The results showed that: (1) 6 genes were expressed significantly and abundantly when Arabidopsis seeds just germinated compared to 5dayold seedlings; during the vegetative growth stage, the signal of At5G42910 gene was not detected, and the remaining 8 genes were detected as the seedlings grew. The relative content of mRNA of them were also increased correspondingly, and the increase of AtDPBF2 and AtDPBF4 was the largest; the relative content of ABF3 in 13dayold seedlings was the highest, about 59 times that of ABI5. (2) In the reproductive growth stage, 9 genes are expressed in a small amount in stems and roots, but in large amounts in flowers and seeds, and the expression level of ABI5 is the highest, which is about 122 times and 730 times of that in roots, respectively; ABF2 expression was the highest in leaves, and AtDPBF2 expression was the highest in fruit pods, The expression levels of the two were about 10 and 12 times higher than that in roots; At5G42910 was highly expressed in flowers and fruit pods, which was about 5 times of the expression in other organs. (3) After being treated with 100 mmol·L^-1 NaCl, 50 μmol·L^-1 sorbitol, and 10 μmol·L^-1 ABA for 8 days, the growth and development of seedlings in each treatment was significantly slower than that of the control, and the inhibitory effect was increased as the treatment concentration increased further. (4) After 13dayold Arabidopsis seedlings were treated with 20 μmol·L^-1 ABA, the relative mRNA contents of ABF1 and ABI5 genes increased the most, and the increase of both was close to 30 times; after 100 mmol·L^-1 sorbitol treatment, the relative mRNA contents of ABF1 and ABI5 genes increased the most, and the increase rates of the two were close to 120 times and 30 times, respectively; 100 mmol·L^-1 NaCl treatment resulted in the downregulation of most ABI5 subfamily genes, but only the ABF3 gene was significantly upregulated; low temperature treatment at 4 ℃ increased the relative mRNA contents of ABF1 and ABI5 genes by about 110 times and 25 times, respectively. These results indicate that the expression of 8 members of the ABI5 subfamily increases gradually during the vegetative growth stage, and the expression of each gene rises sharply after entering the reproductive growth stage, and they are tissue differences. 9 genes are expressed in large quantities in flowers and seeds, but low in roots and stems. ABFs genes in leaves play a major role, and ABI5 and AtDPBF2 genes play a major role in seed formation and maturation; in response to abiotic stress, ABF1 and ABI5 mainly respond to ABA and osmotic stress, and ABF3 mainly responds to salt stress. In addition to ABF1, ABI5 also plays an important role in response to cold stress. It is speculated that At5G42910 gene plays an important role in seed formation and maturation.