参与调控桂花花朵开放的SPL基因鉴定及表达分析
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引用本文:吴秀怡,田清尹,杨秀莲,岳远征,王良桂.参与调控桂花花朵开放的SPL基因鉴定及表达分析[J].西北植物学报,2021,41(11):1801~1809
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作者单位
吴秀怡,田清尹,杨秀莲,岳远征,王良桂* (南京林业大学 风景园林学院南京 210037) 
基金项目:国家自然科学基金(31870695,32071828);
中文摘要:SPL(SQUAMOSA promoter binding protein like)是植物特有的基因家族,在花发育过程中具有重要调控作用。该研究以桂花全基因组数据为基础,对SPL基因家族成员的蛋白理化性质、系统进化、基因结构和不同组织的表达模式进行生物信息学分析,筛选出花组织中较高表达的基因进行实时荧光定量、亚细胞定位及酵母自激活验证,为解析SPL基因参与调控桂花花朵开放过程中的作用机制和功能提供基因资源。结果显示:(1)共鉴定出29个桂花SPL基因家族成员,它们均具有SBP结构域,且不均匀分布在15条染色体上。(2)与拟南芥构建系统进化树,聚类可分为8大亚群,同亚群的OfSPL基因具有高度相似的基因结构。(3)RNA seq数据分析表明,OfSPL9/10/17/19/21/27/28整体FPKM值较高,在花组织中具有一定的特异性;qRT PCR分析表明,OfSPL10/21在花朵开放过程中的相对表达量呈先升后降的趋势。(4)亚细胞定位和转录自激活活性分析显示,OfSPL10/21编码蛋白主要定位于细胞核上,不具有转录自激活活性。研究推测,OfSPL10/21可能参与调控桂花花色与花香物质的生物合成。
中文关键词:桂花  SPL基因  生物信息学  花朵开放  亚细胞定位  酵母自激活
 
Identification and Analysis of SPL Genes in the Regulation of Flower Opening in Sweet Osmanthus
Abstract:SPL (squamosa promoter binding protein like) is a kind of gene family unique to plants. It plays an important role in the process of flower development. The putative OfSPLs were explored and identified from Osmanthus fragrans transcriptome data and their physicochemical properties, phylogeny, gene structure and expression pattern were analyzed. The key genes involved in the process of flower development were obtained by homologous sequence alignment qRT PCR, subcellular localization and yeast self activation. We further provided genetic resources for the mechanism of action and function of the corresponding transcription factors of O. fragrans in flower development. The results showed that: (1) 29 putative OfSPL were confirmed, they are distributed uniformly on 15 chromosomes with SBP domains. (2) According to the phylogenetic tree with Arabidopsis thaliana, they were divided into 8 subfamilies, of which have relatively conservative gene structures. (3) RNA Seq data analysis showed thatOfSPL9/10/17/19/21/27/28 had higher FPKM values and had certain specificity in flower tissues. qRT PCR analysis showed that the OfSPL10/21 expression level generally exhibited an upward trend followed by a downward trend. (4) The subcellular localization analysis showed that OfSPL10/21 proteins of O. fragrans localized in the nucleus, and OfSPL10/21 had no self activating activity and toxicity. The study found that OfSPL10/21 may participate in the synthesis and regulation of color and aroma during the flowering of O. fragrans.
keywords:Osmanthus fragrans  SPL family  bioinformatics evolutionary analysis  flower development  subcellular localization  yeast self activation
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