小麦三雌蕊性状的遗传分析与连锁标记开发
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引用本文:侯光生,闫贵云,李 欣,乔麟轶,常利芳,张晓军,畅志坚.小麦三雌蕊性状的遗传分析与连锁标记开发[J].西北植物学报,2021,41(11):1818~1824
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作者单位
侯光生,闫贵云,李 欣,乔麟轶,常利芳,张晓军,畅志坚* (山西农业大学 农学院/作物遗传与分子改良山西省重点实验室山西农业大学 农学院山西太谷 030801) 
基金项目:山西省重点研发计划项目(201903D211003, 201803D221018 5);
中文摘要:CH257是‘三粒小麦’与普通小麦‘石优20’回交3次后选育的一个三雌蕊材料,可大幅度增加小麦的穗粒数。为了解析小麦三雌蕊性状的遗传方式,挖掘其控制基因并开发相关分子标记,该研究利用CH257和‘中国春’配制组合,获得其F1、F2和BC1F1群体进行遗传分析;使用纯合正常和纯合三雌蕊F2株系,采用分离群体分组分析法(bulked segrgant analysis,BSA)构建单雌蕊、三雌蕊混池进行90K基因芯片扫描,根据芯片结果在相应区段开发分子标记。结果表明:CH257中三雌蕊的发育受1对显性单基因Pis CH257控制;使用90K基因芯片扫描将Pis CH257定位于2DL染色体上,在2DL相应区段开发标记,共获得5个与Pis CH257连锁的共显性SSR标记,其顺序为2DL07、2DL17、2DL22、Pis CH257、2DL25、2DL38,Pis CH257两侧的连锁标记2DL22、2DL25与其遗传距离分别为1.1cM和2.5cM。本研究结果为克隆控制小麦三雌蕊性状的基因奠定了基础。
中文关键词:小麦  三雌蕊  遗传分析  定位  基因芯片
 
Genetic Analysis and Linkage Marker Development of Three pistil Characters in Wheat
Abstract:CH257 is a three pistil material bred after three backcrosses between ‘three grain wheat’ and common wheat ‘Shiyou 20’, which can greatly increase the number of grains per ear. In order to analyze the inheritance of three pistil trait in wheat, and dig out its control genes and develop related molecular markers, this study used CH257 and ‘China Spring’ to formulate a combination, and obtained its F1, F2 and BC1F1 populations for genetic analysis; using homozygous normal and homozygous three pistil F2 lines, bulked segregant analysis (BSA) was used to construct single pistil and three pistil mixed pools for 90K gene chip scanning, and molecular markers were developed in the corresponding segments based on the chip results. The results show that the development of the three pistils in CH257 is controlled by a pair of dominant single gene Pis CH257. We located Pis CH257 on the 2DL chromosome, and develop 5 markers in the corresponding segment of 2DL. The sequence of CH257 linked co dominant SSR markers is 2DL07, 2DL17, 2DL22, Pis CH257, 2DL25 and 2DL38. the linkage markers 2DL22 and 2DL25 are on both sides of Pis CH257 and their genetic distances are 1.1 cM and 2.5 cM, respectively. This study provides a basis for cloning the gene that control the three pistil trait of wheat.
keywords:wheat  three pistil  genetic analysis  location  gene chip
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