Based on the transcriptome sequencing of aborted female flower in Cucurbita moschata, it is suggested that the CmNPR1 may play an important role in flower development of C. moschata. In this study, we used homology cloning method to obtain the CDS of CmNPR1 from the inbred line‘31’. In order to provide a research foundation for further study on the function and mechanism of CmNPR1 in flower development of C. moschata, we analyzed the bioinformatics, expression characteristics and subcellular localization of CmNPR1. The results showed that: (1) the length of the coding sequence of CmNPR1 is 1 442 bp, and it encodes a 480 amino acidlong protein. The protein sequence contains a BTB/POZ and an ankyrin repeats (ANK), and it has no signal peptide and transmembrane structure. The results of multiple sequence alignment showed that the CmNPR1 have the closest genetic relationship with Cucurbita pepo (96.05%), followed by Cucurbita maxima (95.63%). (2) The expression level of CmNPR1 was the highest at the minimum flower developmental stage (0.5 cm), and the expression level in stigma was the highest among different flower structures. (3) The subcellular localization analysis showed that the CmNPR1 was localized to the cytoplasm and nucleus.