一氧化氮对苹果果实愈伤苯丙烷代谢的影响及生理机制分析
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国家自然科学基金中以国际合作与交流项目(31861143046)


Effect of Nitric Oxide on Phenylpropanoid Metabolism in Healing of Apple Fruit and Analysis of Its Physiological Mechanism
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    摘要:

    以“红星”苹果果实为试材,采用0.5 mmol/L的NO供体硝普钠(SNP)溶液浸泡人工损伤的苹果5 min,考察SNP处理后愈伤期间果实失重率和病情指数,分析伤口处苯丙烷代谢关键酶活性和产物含量,以及H2O2含量和过氧化物酶活性,并探讨其相关生理机制,为苹果果实采后快速愈伤提供方法及理论依据。结果表明:(1)SNP处理显著降低了愈伤期间损伤苹果果实的失重率和扩展青霉(Penicillium expansum)损伤接种果实的病情指数,处理果实的失重率和病情指数于处理第5天时较对照分别显著降低40.2%和31.4%。(2)SNP处理显著提高了果实伤口处苯丙烷代谢关键酶苯丙氨酸解氨酶、肉桂酸4羟化酶和肉桂醇脱氢酶的活性,并提高了肉桂酸、咖啡酸、阿魏酸、芥子酸、总酚、类黄酮、P香豆醇、松柏醇、芥子醇及木质素的含量水平;SNP处理显著提高了果实伤口处的H2O2含量以及POD活性。研究发现,NO可通过激活苹果果实伤口处苯丙烷代谢,提高H2O2含量以及POD活性来促进苹果果实的愈伤。

    Abstract:

    Apple fruits of the ‘cv. Starkrimson’ were artificially wounded, dipped with sodium nitroprusside (SNP) as an exogenous donor of NO at 0.5 mmol/L for 5 minutes. To investigate the weight loss and disease index of apple fruits during healing by SNP treated, we analyzed the activities of key enzymes and content of products in phenylpropanoid metabolism, H2O2 content and peroxidase activity at wound sites, and explored its relevant physiological mechanism, so as to provide a method and theoretical basis for rapid postharvest healing of apple fruits. The results indicated that: (1) SNP treatment effectively reduced the weight loss and disease index of inoculated fruits with Penicillium expansum during healing, which were 40.2% and 31.4% lower than the control at the 5th day after healing. (2) The treatment significantly increased the activities of phenylalanine ammonia lyase, cinnamic acid4hydroxylase and cinnamyl alcohol dehydrogenase, the key enzymes of phenylpropanoid metabolism, and enhanced the contents of cinnamic acid, coffeic acid, ferulic acid, sinapic acid, total phenols, flavonoids, Pcoumaroyl alcohol, coniferyl alcohol, sinapyl alcohol and lignin at wound sites of fruits. SNP treatment also significantly raised H2O2 content and POD activity at wound sites of fruits. It was found that NO could promote the wound healing of apples by activating phenylpropanoid pathway, increasing H2O2 content and POD activity at wound sites.

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柴秀伟,孔 蕊,李宝军,等.一氧化氮对苹果果实愈伤苯丙烷代谢的影响及生理机制分析[J].西北植物学报,2022,42(4):619-627

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  • 在线发布日期: 2022-05-18
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