Abstract:NADPmalate enzyme (NADPME) is a key photosynthetic enzyme in C4 plants and plays an important role in biotic and abiotic stress. To further study the function of NADPME, we cloned the NADPME family gene sequences from the leaves of C3C4 species Salsola laricifolia, and then determined the expression patterns in tissues under different abiotic stresses. We also cloned NADPMEs promoter sequences and analyzed the element differences in response to abiotic stress based on bioinformatics software. The results showed that: (1) three NADPMEs were obtained, named SaNADPME1, SaNADPME2 and SaNADPME4, with the length of CDS sequence was 1 755 bp, 1 758 bp and 1 941 bp, respectively. (2) SaNADPME1 was mainly expressed in roots, while SaNADPME2 and SaNADPME4 were mainly expressed in leaves. Under the stress of ABA, NaCl, NaHCO3 and PEG6000, three NADPMEs could be induced to express in Salsola laricifolia, and the response expression patterns of SaNADPME4 and SaNADPME2 were similar. (3) The length of promoter regions of SaNADPME1, SaNADPME2, and SaNADPME4 were 2 351 bp, 1 655 bp and 2 887 bp. Bioinformatics analysis showed that they contained not only basic promoter elements, but also elements in response to stress response.