| 水杨酸对干旱胁迫下西洋参不定根生化指标及药用成分含量的影响 |
| 投稿时间：2022-08-29 修订日期：2023-06-09 点此下载全文 |
| 引用本文：卢超,陈景鲜,王国霞,等.水杨酸对干旱胁迫下西洋参不定根生化指标及药用成分含量的影响[J].西北植物学报,2023,43(8):1295~1303 |
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| 基金项目:国家自然科学基金项目（32000235）；河南省重点高校科研项目（22B360019）;郑州师范学院博士科研基金（501413）；郑州师范学院大学生科研创新基金项目（2021012） |
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| 中文摘要:本研究以西洋参不定根为材料，使用质量分数为10%的PEG-6000进行模拟干旱胁迫预处理，添加不同浓度（0、10、40和80 μmol·L-1）的外源水杨酸（SA）分别处理2、4、6、8 d，检测西洋参不定根中抗氧化酶活性，渗透调节物质含量，药用成分总黄酮、总皂苷、单体皂苷Rb1和Re的积累量，及两个bHLH转录因子和人参皂苷生物合成途径中6个关键酶基因的表达量，探究外源SA响应西洋参模拟干旱胁迫的调节机制。结果显示：（1）过氧化氢酶（CAT）和过氧化物酶（POD）活性均在80 μmol·L-1的SA处理4天时出现峰值。游离脯氨酸的含量在80 μmol·L-1的SA处理4天时达到峰值，丙二醛（MDA）的含量在40 μmol·L-1的SA处理到6天时达到峰值。（2）总黄酮的含量在80 μmol·L-1的SA处理6天时达到最大值，总皂苷和单体皂苷Re含量在40 μmol·L-1的SA处理8天时达到最大值，单体皂苷Rb1含量在40 μmol·L-1的SA处理到第6天时达到最大值。（3）HMGR和DS基因表达量在40 μmol·L-1的SA处理第2天时提高了3倍以上，Pq-CYP6H、Pq-D12H、Pq-3-O-UGT1和Pq-bHLH25基因表达量在SA处理后提高了1倍以上。研究表明，适宜浓度的外源SA可以改善西洋参不定根在干旱胁迫环境下的生长状态指标，提高有效成分含量和相关基因的表达量。 |
| 中文关键词:西洋参 干旱胁迫 水杨酸 生化指标 药用成分 |
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| Effects of Salicylic acid on Biochemical Indexes and Contents of Medicinal Components in Adventitious Roots of Panax quinquefolium under Drought Stress |
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| Abstract:In this study, the adventitious root of Panax quinquefolium L. was used as the material. The simulated drought stress was pretreated with 10% PEG-6000, and then exogenous salicylic acid (SA) at different concentrations (0, 10, 40, and 80 μmol·L-1) were added to treat 2, 4, 6 and 8 days, respectively. Then, the activity of antioxidant enzymes, the content of osmotic regulators, the accumulation of total flavonoids, total saponins, monomer saponins Rb1 and Re, gene expression of two bHLH transcription factors and 6 key enzymes in the ginsenosides biosynthetic pathway in adventitious roots of P. quinquefolium were detected. These results were used to explore the regulatory mechanism of exogenous SA in response to simulated drought stress in P. quinquefolium. The results showed that: (1) The activities of catalase (CAT) and peroxidase (POD) both peaked when 80 μmol·L-1 of SA was treated for 4 days. The content of free proline reached the peak at 4 days after 80 μmol·L-1 of SA treatment, and the content of malondialdehyde (MDA) reached the peak at 6 days after 40 μmol·L-1 of SA treatment. (2) The content of total flavonoids reached the maximum value when 80 μmol·L-1 of SA was treated for 6 days, and the content of total saponins and monomeric saponins Re reached the maximum value when treated with 40 μmol·L-1 of SA for 8 days, the content of monomeric saponin Rb1 reached the maximum value at the 6 days of 80 μmol·L-1 of SA treatment. (3) The expression levels of HMGR and DS genes were increased by more than 3 times on the second day after 40 μmol·L-1 of SA treatment, and the gene expressions of Pq-CYP6H, Pq-D12H, Pq-3-O-UGT1 and Pq-bHLH25 The amount was more than doubled after SA treatment. These experimental results showed that the appropriate concentration of exogenous SA could improve the growth state indicators of adventitious roots of P. quinquefolium under drought stress, and increase the content of active ingredients and the expression of related genes. |
| keywords:Panax quinquefolium L. drought stress salicylic acid biochemical index medicinal ingredient |
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