丛枝菌根真菌对盐碱胁迫下杜梨幼苗生长和生理特性的影响
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引用本文:龚远博,胡吉怀,胡丁猛,许景伟,王延平.丛枝菌根真菌对盐碱胁迫下杜梨幼苗生长和生理特性的影响[J].西北植物学报,2022,42(8):1320~1329
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作者单位
龚远博1,胡吉怀1,胡丁猛2,许景伟2,王延平1* (1 山东农业大学 林学院山东泰安 2700182 山东省林业科学研究院济南 250014) 
基金项目:山东省农业良种工程课题“杜梨优良种质资源收集保存和创新利用”(2019LZGC009 3)
中文摘要:采用盆栽实验,设置梯度盐碱环境(0、1.5、3.0、4.5、6.0、7.5 g·kg-1),接种根内根孢囊霉(Rhizophagus intraradices,Ri),对比分析杜梨幼苗苗高生长、叶片光合气体交换参数和叶绿素荧光参数及抗逆生理指标的变化,探讨在混合盐碱胁迫下接种菌根真菌对杜梨幼苗生长和抗逆性的影响,为盐碱地杜梨菌根化栽培提供理论依据。结果表明:(1)根内根孢囊霉能够与杜梨根系形成良好的共生,但在盐碱浓度6.0 和7.5 g·kg-1时侵染率分别下降为47.98%和32.97%。(2)盐碱胁迫条件下,接种Ri显著提高了杜梨苗高生长量和生物量,同时显著提高了杜梨叶片光合色素的含量和净光合速率;尤其在高盐碱程度(6.0 g·kg-1、7.5 g·kg-1)下显著提高了气孔导度和胞间二氧化碳含量,有利于光化学淬灭系数提高和非光化学淬灭系数的降低,促进实际光化学效率提升。(3)接种Ri显著降低了杜梨幼苗叶片细胞膜透性和丙二醛的含量,显著增强了超氧化物歧化酶和过氧化物酶活性,并提高了可溶性糖和可溶性蛋白含量。研究发现,接种根内根孢囊霉能显著增强杜梨在盐碱胁迫下的抗氧化酶活性和渗透调解能力,有效提升其生长量和光合效能,缓解盐碱胁迫对杜梨造成的损伤;实施杜梨幼苗菌根化可有效提升苗木在盐碱地的生长和抗逆性能,有利于其在盐碱地综合治理中发挥更大作用。
中文关键词:杜梨  丛枝菌根真菌  盐碱胁迫  光合特性  生理特性
 
Effects of Arbuscular Mycorrhizal Fungi on the Growth and Physiological Traits of Pyrus betulifolia under Salt alkali Stress
Abstract:Pot experiment was conducted to set up gradient saline alkali environment (0, 1.5, 3.0, 4.5, 6.0 and 7.5 g·kg-1) and inoculate Rhizophagus intraradices (Ri). The changes of seedling height growth, leaf photosynthesis, chlorophyll fluorescence parameters and stress resistance physiological indexes of Pyrus betulifolia seedlings were compared and analyzed. We studied the effects of arbuscular mycorrhizal fungi inoculated with mixed saline alkali on the growth and stress resistance of P. betulifolia seedlings to provide a theoretical basis for mycorrhizal P. betulifolia afforestation in saline alkali environment. The results show that: (1) R. intraradices could form a good symbiosis with the root system of P. betulifolia, but the infection rate decreased to 47.98% and 32.97% at 6.0 g·kg-1and 7.5 g·kg-1 saline alkali concentration, respectively. (2) Under saline alkali stress, Ri inoculation significantly increased height growth and biomass, photosynthetic pigment content and net photosynthetic rate of P. betulifolia leaves. Especially at high saline alkali concentration (6.0 g·kg-1and 7.5 g·kg-1), RI inoculation significantly increased the stomatal conductance and intercellular carbon dioxide content of P. betulifolia leaves, which was conducive to the increase of photochemical quenching coefficient and the decrease of non photochemical quenching coefficient, and promoted the improvement of actual photochemical efficiency. (3) Ri inoculation significantly decreased the cell membrane permeability and the content of MDA, significantly increased the activities of superoxide dismutase and peroxidase, and increased the contents of soluble sugar and soluble protein in the leaves of P. betulifolia seedlings. It was found that inoculation with R. intraradices could significantly enhance the activities of antioxidant enzymes and osmotic mediation ability of P. betulifolia under saline alkali stress, effectively enhance its growth and photosynthetic efficiency, and finally alleviate the damage caused by saline alkali stress. Therefore, the implementation of mycorrhizalization of P. betulifolia seedlings can effectively improve the growth and stress resistance of seedlings in saline alkali soil, which is beneficial to play a greater role in the comprehensive management of saline alkali soil.
keywords:Pyrus betulifolia  arbuscular mycorrhizal fungi (AMF)  saline alkali stress  photosynthetic characteristics  physiological characteristics
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