NaHCO3胁迫对三色堇生长及抗逆生理特性的影响
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引用本文:刘小娥,苏世平.NaHCO3胁迫对三色堇生长及抗逆生理特性的影响[J].西北植物学报,2022,42(8):1339~1346
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作者单位
刘小娥,苏世平* (甘肃农业大学 林学院兰州 730070) 
基金项目:国家自然科学基金(32060335);
中文摘要:以三色堇实生苗为材料,设置不同浓度的NaHCO3[0(CK)、25、50、100、150和200 mmol·L-1]处理,在胁迫后第7天和第14天分别测定各处理幼苗叶片的渗透调节物质含量、抗氧化酶活性、叶绿素含量、丙二醛(MDA)含量以及株高生长量,探讨三色堇对NaHCO3胁迫的生理响应机制。结果表明:(1)三色堇株高生长量在NaHCO3浓度小于等于50 mmol·L-1时较CK显著增加,在100 mmol·L-1时与CK相近,在大于100 mmol·L-1时较CK显著降低。(2)各浓度处理三色堇叶片可溶性糖(SS)、可溶性蛋白(SP)、游离脯氨酸(Pro)含量在胁迫第7天均显著高于CK;而胁迫第14天时,各浓度处理的SS含量、50 mmol·L-1处理的SP含量以及150、200 mmol·L-1处理的Pro含量仍显著高于CK。(3)胁迫第7天时,三色堇叶片超氧化物歧化酶(SOD)活性在各胁迫浓度下较CK均显著增强,但其过氧化物酶(POD)、过氧化氢酶(CAT)活性均无显著变化;胁迫第14天时,各浓度处理的CAT活性、50 mmol·L-1处理的POD活性以及100、150 mmol·L-1处理的SOD活性均显著高于相应CK。(4)在NaHCO3胁迫过程中,三色堇叶片MDA含量均随着胁迫浓度增加而逐渐增加,且均显著高于CK。(5)三色堇叶片的叶绿素含量在胁迫第7天时均无显著变化,胁迫第14天时也仅在150或者200 mmol·L-1处理下较CK显著降低。研究发现,三色堇植株能耐受小于等于100 mmol·L-1NaHCO3胁迫,NaHCO3对株高生长有低浓度促进、高浓度抑制的剂量效应;三色堇在NaHCO3胁迫期间能够通过增加渗透调节物质含量、增强抗氧化酶活性来缓解胁迫诱导的过氧化伤害,一定程度上提高了幼苗的耐受能力。
中文关键词:三色堇  NaHCO3胁迫  生长量  渗透调节物质  抗氧化酶活性  叶绿素含量
 
Growth and Physiological Characteristics of Stress Resistance in Seedling of Viola tricolor under NaHCO3 Stress
Abstract:In order to explore the growth and physiological characteristics of stress resistance in seedling of Viola tricolor under NaHCO3 stress, we treated the seedlings of V. tricolor with different concentrations of NaHCO3 [0 mmol·L-1 (CK), 25 mmol·L-1, 50 mmol·L-1, 100 mmol·L-1, 150 mmol·L-1, 200 mmol·L-1], and determined the height growth, osmotic regulation substances, antioxidant enzyme activity, photosynthetic pigment content, and malondialdehyde (MDA) content on the 7th day and 14th day. The results showed that: (1) compared with CK, the height growth of V. tricolor was significantly increased when NaHCO3 concentration was less than 50 mmol·L-1, and close to CK when NaHCO3 concentration was 100 mmol·L-1, but significantly decreased when NaHCO3 concentration was higher than 100 mmol·L-1. (2) On the 7th day, the contents of soluble sugar (SS), soluble protein (SP) and free proline (Pro) in V. tricolor leaves were significantly higher under NaHCO3 treatments than under CK, while on the 14th day, the contents of SS under all NaHCO3 concentration, SP under 50 mmol·L-1, Pro under 150 and 200 mmol·L-1 were significantly higher than under CK. (3) On the 7th day, the activity of superoxide dismutase (SOD) in V. tricolor leaves was significantly increased under NaHCO3 treatments than under CK, while the activities of peroxidase (POD) and catalase (CAT) had no significant change. On the 14th day, the contents of CAT under all NaHCO3 concentration, POD under 50 mmol·L-1, SOD under 100 and 150 mmol·L-1 were significantly higher than under CK. (4) MDA content in V. tricolor leaves increased gradually with the increase of NaHCO3 concentration, and was significantly higher than CK. (5) Compared with CK, chlorophyll content in V. tricolor leaves did not change significantly on the 7th day, while decreased significantly under 150 or 200 mmol·L-1 NaHCO3 concentration on the 14th day. The results indicated that V. tricolor could tolerate lower than 100 mmol·L-1NaHCO3 concentration and NaHCO3 had a dose effect of promoting plant height growth at low concentration and inhibiting plant height growth at high concentration. V. tricolor could alleviate the peroxidation damage induced by NaHCO3 stress through increasing the contents of osmotic regulatory substances and enhancing the activities of antioxidant enzymes, and thus improved the tolerance of V. tricolor seedlings.
keywords:Viola tricolor  NaHCO3 stress  plant growth  osmotic regulation substances  antioxidant enzyme activity  chlorophyll content
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