| 基于表型及经济性状构建蒜头果初选核心种质 |
| 投稿时间：2022-09-28 修订日期：2023-07-05 点此下载全文 |
| 引用本文：李洪果,段润梅,覃坚,等.基于表型及经济性状构建蒜头果初选核心种质[J].西北植物学报,2023,43(8):1396~1404 |
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| 基金项目:广西自然科学基金项目（2020GXNSFAA297261）；中国林业科学研究院热带林业实验中心科学基金项目（RL-2017-02） |
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| 中文摘要:通过构建蒜头果核心种质,对天然分布的蒜头果资源进行评价和利用,明确资源收集保护的优先对象,并为建立高含油率和高神经酸含量蒜头果育种群体提供备选材料。将97份蒜头果种质按地理区域分组,通过检测叶片、果实、种仁及其内含物等21个性状,在系统聚类和优先取样法的基础上,根据遗传多样性法、比例法和对数法3种方法,设定10%、20%和30%3种取样比例,共产生7种取样策略。采用极差符合率(CR)、平均多样性指数(MI)、最大值变化率(CRMAX)、最小值变化率(CRMIN)、变异系数变化率(VR)、均值差异百分率(MD)、方差差异百分率(VD)和平均值变化率(CRMEA)等8个参数评价7种策略构建的核心种质,从中选出参数最优的核心种质。通过比较核心种质与原种质的表型多样性指数、符合率、主成分和基于主成分的样品分布图,验证所构建核心种质的代表性。在类平均法聚类的基础上,“优先取样法+遗传多样性法+30%取样比例”形成的核心种质参数最优,前述8个评价参数分别为：98.22%、1.96、99.16%、100.82%、123.14%、0.00、14.29%和100.70%,核心种质包含28个样本,最终取样比例为28.87%。核心种质与原种质在21个性状上的多样性指数经t检验,差异均不显著。核心种质与原种质在21个性状上的均值、极大值、极小值和多样性指数的平均符合率为：97.31%、99.16%、99.18%和95.41%。原种质和核心种质6个主成分的累积贡献率分别为：86.64%和87.78%,两者基于主成分的样品分布图具有相似的分布结构。构建的蒜头果核心种质具备代表性、有效性等特征,可作为蒜头果种质资源收集保存和建立育种群体等的依据。 |
| 中文关键词:蒜头果 核心种质 表型性状 种质资源 |
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| Construction of primary core collection of Malania oleifera based on Phenotypic and economic traits |
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| Abstract:By constructing the core collection of Malania oleifera, the natural distribution of Malania oleifera resources were evaluated and utilized, the priority objects of resource collection and protection will be identified, and the alternative materials will be provided for establishing the breeding population of Malania oleifera with high oil content and high neuritic acid content. 97 Malania oleifera germplasms were grouped according to geographical regions, and 21 traits including leaves, fruits, kernels and their contents were detected. On the basis of systematic clustering and preferential sampling method, according to genetic diversity method, proportional method and logarithmic method, three sampling ratios of 10%, 20% and 30% were set, resulting in a total of seven sampling strategies. Eight parameters such as CR, MI, CRMAX, CRMIN, VR , MD, VD and CRMEA were used to evaluate the core collection constructed by the seven strategies, and the core collection with the best parameters was selected. The representativeness of the constructed core collection was verified by comparing the phenotypic diversity index, coincidence rate, principal components and sample distribution map between the core collection and the initial collection. On the basis of clustering by class average method, the core collection parameters formed by "priority sampling method + genetic diversity method +30% sampling ratio" were the best. The above eight evaluation parameters were 98.22%, 1.96, 99.16%, 100.82%, 123.14%, 0.00, 14.29% and 100.70%, respectively. The core collection contained 28 samples, and the final sampling proportion was 28.87%. There was no significant difference in the diversity index of 21 traits between the core collection and the initial collection by t test. The average coincidence rates of mean, maximum, minimum and diversity index of 21 traits between the core collection and the initial collection were 97.31%, 99.16%, 99.18% and 95.41%. The cumulative contribution rates of the six principal components of the initial and core collection were 86.64% and 87.78%, respectively. The distribution maps based on principal components of the initial and core collection had similar distribution structures. The core collection of Malania oleifera constructed has the characteristics of representativeness, validity and practicability, which can be used as the basis for the collection and preservation of Malania oleifera germplasm resources and the establishment of breeding population. |
| keywords:Malania oleifera , core collection, phenotypic traits , germplasm |
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