| 苯丙烷代谢基因差异表达对宁夏枸杞适应盐胁迫的调控作用 |
| 投稿时间：2022-11-30 修订日期：2023-05-12 点此下载全文 |
| 引用本文：宋 繁,胡进红,梁旺利,等.苯丙烷代谢基因差异表达对宁夏枸杞适应盐胁迫的调控作用[J].西北植物学报,2023,43(8):1286~1294 |
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| 基金项目:宁夏自然科学(2022AAC03083)；宁夏回族自治区科技创新团队建设项目(NXKJT2019002) |
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| 中文摘要:为探究苯丙烷代谢在宁夏枸杞适应盐胁迫中的作用机制。本研究以不同浓度NaCl（0，100，200，300 mmol/L NaCl）处理的宁夏枸杞为研究对象，利用高通量测序技术和qRT-PCR检测分析盐胁迫下宁夏枸杞苯丙烷代谢相关基因差异表达规律，同时对关键酶的活性及相关物质含量进行测定。结果表明：（1）宁夏枸杞在不同浓度NaCl处理下共有58个苯丙烷代谢相关基因差异表达，且随着盐胁迫程度的增加大部分基因表达呈上调或不变；（2）随着NaCl浓度的增加，宁夏枸杞叶片抗氧化酶SOD、POD、CAT的活性均下降，而酚类物质、类黄酮和木质素的含量在100 mmol/L NaCl处理下显著积累。结果表明宁夏枸杞可能通过调控苯丙烷代谢相关基因上调表达，增加酚类物质、类黄酮和木质素的合成，通过清除过多ROS和提升细胞壁强度以适应盐胁迫。宁夏枸杞可耐受的NaCl浓度在100至200 mmol/L之间。本研究结果为宁夏枸杞栽培生产及今后进一步解析宁夏枸杞的耐盐机制提供理论依据。 |
| 中文关键词:宁夏枸杞 盐胁迫 苯丙烷代谢途径 差异表达基因 酚类物质 类黄酮 木质素 |
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| Regulation of Phenylpropane Metabolism Genes Differential Expression on Adaptation of Lycium barbarum L. to Salt Stress |
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| Abstract:To explore the mechanism of phenylpropane metabolism in adapting to salt stress of L. barbarum. In this study, L. barbarum treated with different concentrations of NaCl (0, 100, 200, 300 mmol/L NaCl) was used as the research object. High-throughput sequencing technology and qRT-PCR were used to detect and analyze the differential expression of genes related to phenylpropane metabolism of L. barbarum under salt stress. Meanwhile, the activities of key enzymes and the contents of related substances were determined. The results showed as follows: (1) A total of 58 genes related to phenylpropane metabolism were differentially expressed under different concentrations of NaCl, and most of the genes were up-regulated or unchanged with the increase of salt stress. (2) With the increase of NaCl concentration, the activities of antioxidant enzymes SOD, POD and CAT in the leaves of L. barbarum decreased, while the contents of phenols, flavonoids and lignin were significantly accumulated under 100 mmol/L NaCl treatment. The results showed that L. barbarum could regulate the up-regulated expression of genes related to phenylpropane metabolism, increase the synthesis of phenols, flavonoids and lignin, and adapt to salt stress by clearing excessive ROS and improving cell wall strength. The concentration of NaCl that L. barbarum can tolerate is between 100 and 200 mmol/L. The results of this study provide theoretical basis for the cultivation and production of L. barbarum and the further analysis of the salt tolerance mechanism of L. barbarum. |
| keywords:Lycium barbarum L. salt stress phenylpropanoid metabolism differentially expressed genes phenolic compounds flavonoids lignin |
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