Abstract:In this study, ABI1 gene was cloned from Lilium oriental hybrid ‘Sorbonne’ by RT-PCR, and the bioinformatics analysis were performed. Meanwhile, the qRT-PCR was used to detect the expression patterns of LoABI1 in lily at different tissues and in the process of low temperature treatment and after transplanting, for clarifying the functional characteristics of LoABI1 gene and lay a foundation for the analysis of the ABA signal transduction pathway and the mechanism of breaking dormancy at low temperature in lily. The results showed that: (1) the LoABI1 gene of lily was cloned successfully. The coding sequence of LoABI1 was 1 341 bp long and LoABI1 encoded 446 amino acids containing a conserved phosphatase 2C (PP2C) domain. (2) Phylogenetic analysis showed that LoABI1 was closest to Oryza sativa and clustered with AtABI1, which is a member of Arabidopsis thaliana PP2C gene family, and both of them belong to PP2C A group. (3) The subcellular localization results showed that LoABI1 is localized in the nucleus and cytoplasm of tobacco epidermal cells. (4) Real time RT-PCR expression analysis showed that LoABI1 expressed in stem root, tender stem, leaf, and floral organ, and the expression level is higher in tender tissues; The expression of LoABI1 increased at first reaching the peak at the fifth week of cold storage, and then decreased during cold storage. Finally, it remained at a low-level during colonization. (5) The lily bulbs treated at 4 ℃ for 60 days could germinate at 14-28 days, and the buds could be seen at 28-48 days after transplanting, while the bulbs without low temperature treatment neither germinated nor flowered. It is speculated that LoABI1 gene may play an important role in the process of breaking bulbs dormancy at low temperature, and LoABI1 protein may play an important role in lily ABA signal transduction.