Metal tolerance protein (MTP) is an important member of cation transporter family (CDF), which plays a key role in heavy metal transport and detoxification. In order to systematically identify and analyze the cucumber MTP gene family (CsMTP), this study used the MTP sequence of model species as seed sequence to BLASTP the amino acid sequence database of cucumber, and successfully identified 10 CsMTP candidates. Then phylogenetic tree, conservative domain, gene structure, chromosome location and other biological analysis were carried out. The results show that 10 CsMTPs can be divided into Fe / Zn-CDF (2) Mn-CDF (3) and Zn-CDF subfamily (5), which were further named as CsMTP3 to CsMTP12 based on phylogenetic relationship. Most CsMTPs contain 6 transmembrane domains (TMD). CsMTP8, CsMTP9 and CsMTP11, which belong to Mn-MTP subgroup, contain the same sequence DxxxD, HxxxD motifs were observed in CsMTP3, 4, 5 and CsMTP7 of Zn-MTP and Fe / Zn MTPs subgroups. CsMTPs showed tissue and treatment-specific expression patterns. RNA-seq analysis revealed that CsMTP11, CsMTP3 and CsMTP7 maintained relatively higher expression level. qRT-PCR study demonstrated that CsMTPs could be induced by the treatment of different metals, including metals that are not the potential substrate of MTP family.