不同浓度Fe2+、Zn2+对羊草幼苗生长和生理特性的影响
投稿时间:2021-05-10  修订日期:2021-09-09  点此下载全文
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作者单位邮编
孟令博 内蒙古大学 010021
赵曼 内蒙古大学 010021
亢燕 内蒙古大学 010000
祁智 内蒙古大学 010021
基金项目:内蒙古自治区科技重大专项“羊草钙营养形成机理研究与优化技术集成示范”(zdzx2018016);国家自然科学基金“羊草锌稳态调控的分子机理研究”(32060063)
中文摘要:以羊草幼苗为研究对象,通过调整全营养培养基(CK, 0.05 mmol/L Fe2+,0.015 mmol/L Zn2+)中铁或者锌含量设置0、10倍、20倍Fe2+(Zn2+)浓度处理Fe 0(Zn 0)、Fe 10(Zn 10)、Fe 20(Zn 20),以及在高铁培养基中添加0.15 mmol/L Zn2+或10 mmol/L Ca2+、5 mmol/L Mg2+、20 mmol/L K+处理,测定培养6d后幼苗生长指标和矿质元素含量,以及高铁(Fe 20)处理下幼苗根中抗氧化指标和相关基因表达量,探究不同浓度Fe2+、Zn2+对羊草幼苗生长、矿质元素吸收积累及抗氧化指标、基因表达的影响。结果表明:(1)缺锌(Zn 0)显著抑制羊草幼苗鲜重的增加和Zn元素的积累,而促进Fe、Mg元素的积累;高浓度锌(Zn 10、Zn 20)显著促进幼苗叶片生长和Zn元素的积累;缺铁(Fe 0)显著抑制幼苗的根长、鲜重和Fe元素的积累,促进Mg、Zn元素的积累;高浓度铁(Fe 10、Fe 20)显著抑制羊草幼苗根叶生长、根毛发育和Ca、Zn、Mg、K元素的积累;(2)增加Zn2+和Ca2+、Mg2+、K+浓度无法恢复高铁胁迫对幼苗生长的抑制作用。(3)高浓度铁(Fe 20)处理羊草幼苗48 h后,根部过氧化物酶、超氧化物歧化酶、过氧化氢酶、抗坏血酸过氧化物酶、谷胱甘肽还原酶活性和丙二醛、抗坏血酸、还原型谷胱甘肽含量显著升高;烟酰胺合成酶基因、过氧化物酶基因表达量显著下调,植物类萌发素蛋白基因表达量显著上调。研究发现,羊草幼苗生长发育和矿质元素积累对环境中Zn2+浓度变化不敏感,却受到环境中高浓度Fe2+的显著抑制,并造成严重的氧化胁迫伤害,这种伤害在增添Zn2+和Ca2+、Mg2+、K+的条件下无法恢复。
中文关键词:羊草      生理特性
 
The effects of different concentrations of Fe2+ and Zn2+ on growth and physiological characteristics of Leymus chinensis seedlings
Abstract:The Leymus chinensis seedlings as the research object, We set 0, 10 and 20 times Fe2+ (Zn2+) concentration by adjusting the content of iron and zinc in total nutrition medium (CK, 0.05 mmol/L Fe2+, 0.015 mmol/L Zn2+) as Fe 0 (Zn 0), Fe 10 (Zn 10), Fe 20 (Zn 20). And adding 0.15 mmol/L Zn2+ or 10 mmol/L Ca2+, 5 mmol/L Mg2+, 20 mmol/L K+ to the high concentration iron medium. The growth index and mineral element content of seedlings were measured after 6 days of culture, as well as the antioxidant indexes and related gene expression in the roots of seedlings treated with high concentration iron (Fe 20). To explore the effects of different concentrations of Fe2+ and Zn2+ on the growth, mineral element absorption and accumulation, antioxidant index and gene expression of Leymus chinensis seedlings. The results show that: (1) Deficiency of Zn(Zn 0) significantly inhibited the increase of fresh weight and the accumulation of Zn elements, and promoted the accumulation of Fe and Mg elements. High concentration of Zn(Zn 10, Zn 20) significantly promoted the growth of seedling leaves and the accumulation of Zn. Deficiency of Fe(Fe 0) significantly inhibited the root length, fresh weight and the accumulation of Fe elements, and promoted the accumulation of Mg and Zn elements in seedlings. High concentration of Fe(Fe 10, Fe 20) significantly inhibited the root and leaf growth, root hair development and the accumulation of Ca, Zn, Mg and K elements in Leymus chinensis seedlings. (2) Increasing the concentration of Zn2+ and Ca2+, Mg2+, K+ could not restore the inhibitory effect of high concentration iron stress on seedling growth. (3)After treatment of Leymus chinensis seedlings with high concentration of iron (Fe 20) for 48 hours, the activities of peroxidase, superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase and the contents of malondialdehyde, ascorbic acid and reduced glutathione in roots were increased significantly, while the gene expressions of Nicotianamine Synthase and Peroxidase were down-regulated significantly and plant germinin-like protein were up-regulated significantly. The research found that the growth and development of Leymus chinensis seedlings and the accumulation of mineral were not sensitive to the change of Zn2+ concentration in the environment, but were significantly inhibited by the high concentration of Fe2+ in the environment, and caused serious oxidative stress damage, which could not be recovered with the addition of Zn2+ and Ca2+, Mg2+, K+.
keywords:Leymus chinensis  Iron  Zinc  Physiological characteristics
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