储存条件对单瓣和重瓣紫斑牡丹‘象牙白’花粉活力及生理特性的影响
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1.甘肃农业大学林学院;2.甘肃农业大学信息科学技术学院

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甘肃省牡丹工程研究中心(XZ20181120),甘肃省科技厅“甘肃省科技支撑计划项目”(1604NKCA053)


Pollen Activity and Physiological Characteristics of Sing-petal and Double-petal Paeonia Rockii "Xiangyabai" under Different Storage Conditions
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    摘要:

    以紫斑牡丹‘象牙白’单瓣和重瓣花的花粉为材料,对其超微结构及生理生化特性进行观察和分析,探讨单瓣和重瓣花花粉超微结构以及不同温度储存花粉活力与其可溶性蛋白含量、MDA含量和3种保护酶活性(POD、SOD、CAT)的关系,确定紫斑牡丹单瓣和重瓣花花粉最佳储存温度。结果表明:(1)紫斑牡丹重瓣花花粉平均极轴长、平均赤道轴长、极轴长/赤道轴长均小于单瓣花花粉,花粉粒小、瘪粒和畸形是导致其萌发率较低的主要原因。(2)紫斑牡丹单瓣和重瓣花花粉适合在室温条件进行短期储存(1~120d),适合在4℃、-20℃下进行较长时间储存,适合在-80℃下进行长期储存。(3)低温能有效延长花粉储存寿命,抗逆蛋白的形成可有效延缓花粉萌发率的下降,但存储期间花粉细胞膜脂过氧化程度加剧以及细胞损伤加重会迅速导致花粉萌发率降低。(4)低温胁迫对单瓣花花粉的影响作用力低于重瓣花花粉,在相同储存温度条件下,重瓣花花粉细胞受损程度更高、萌发率下降速率更快。

    Abstract:

    The study with" Paeonia rockii "Xiangyabai" single and double pollen as materials, physiological and biochemical characteristics, discussed the relationship between the pollen activity and the activities of protein, MDA and three protective enzymes(POD, SOD, CAT) at different temperatures, determined the best storage mode of single and double pollen. The results show that:(1)The average polar axis length, the average equatorial axis length, and the polar axis length/equatorial axis length of the pollen of the double petal flowers of Paeonia rockii are smaller than those of the single petal flowers. (2)Room temperature is suitable for short-term storage of single-petal and double-flowered pollen for 1~120 days, 4°C and -20°C are suitable for long-term storage of pollen, and -80°C is suitable for long-term storage of pollen. Small, shriveled and deformed pollen grains are the main reasons for the low germination rate. (3)Low temperature can effectively prolong the storage life of pollen grains, and the formation of stress resistant proteins can effectively delay the decline of pollen germination rate. However, during storage, the degree of membrane lipid peroxidation of pollen cells increases, and the aggravation of cell damage will quickly lead to the decline of pollen germination rate. (4)The effect of low temperature stress on single-flowered pollen was lower than that of double-flowered pollen. Under the same storage conditions, the pollen cells of the double-flowered pollen were more damaged and the germination rate decreased faster.

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  • 收稿日期:2022-05-12
  • 最后修改日期:2022-10-19
  • 录用日期:2022-09-26
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