Basic on transcriptome data, we cloned the full length cDNAs of transcription factors genes AmWRKY1like from A. mongolicum by PCR amplification, and their bioinformatic analysis and localization analysis were performed. These will contribute to study the biological functions of AmWRKY1like responding to drought stress in A. mongolicum. The results showed that: AmWRKY1like gene were successful cloned, and the open reading frame (ORF) was 1 182 bp and encoded 393 amino acids, and contains a typical conserved domain of WRKY transcription factors. AmWRKY1like gene was downregulated in leaves of A. mongolicum compared with CK under natural drought and 15% PEG6000 drought conditions via qRTPCR. Subcellular localization assay showed that AmWRKY1like was specifically expressed in the nucleus.