Abstract:Present study use ‘Benibalado’ as the experimental material. In the early stage of véraison, grape clusters were treated with 300 mg/L ABA, water treated as control. The weight, titratable acid and total soluble solids of grape berries at different development stages were determined. Meanwhile, we detected the concentrations of total anthocyanins and endogenous hormone ABA in grape skins. The expression of ABA signaling pathway and anthocyanin biosynthesis related genes in skins of different development stages were observed by qRTPCR. Promoters of 6 anthocyanin biosynthesis related genes were cloned by PCR, the cisacting elements in these promoters were predicted to exploring the mechanism of ABA promoted grape skin coloring and anthocyanin accumulation of ‘Benibalado’ grape at transcriptional regulation level. The results showed that: (1) ABA treatment significantly increased the total soluble solid while decreased titratable acid of ‘Benibalado’ grape. (2) ABA treatment significantly increased the anthocyanin content and endogenous ABA content in the skin of ‘Benibalado’ berries. (3) After ABA treatment, the expression levels of 9 ABA signaling pathway genes and 6 anthocyanin biosynthesis related genes of ‘Benibalado’ grape skins were significantly increased. (4) The results of cisacting element analyzing showed that the promoter sequences of above six anthocyanin biosynthesis related genes contained several ABRE motif which were reported related to ABA signal response. In summary, the results suggested that 9 ABA signaling pathway genes may play important roles in grape skins coloring, two of them (VvABFs) may directly works with the promoter sequence of anthocyanin biosynthesis related genes, which are abundant of ABRE elements to positive regulate the transcription level of downstream genes and eventually modulate the skins coloring and anthocyanin accumulation of grape.