Analysis of Triterpenic Acid Contents in Fruits of 219 Jujube Germplasm Resources
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    Abstract:

    This study aims to make clear the characteristics of triterpenic acid components and contents in fruits of jujube germplasm resources and to provide theoretical reference for the development and utilization of triterpenic acid. The crisp mature fruits of 219 jujube germplasm resources were used as the test materials, by using Ultra High Performance Liquid Chromatography (UPLC) method, the triterpenic acid components including maslinic acid, betulinic acid, oleanolic acid and ursolic acid were determined, and we performed the descriptive statistics, correlation and cluster analysis to screen the excellent resources of higher triterpenic acid contents. The results showed that: (1) the distribution range of total triterpenic acid (TTA) in jujube resources was 95.72-737.82 μg·g-1, and the average content was 306.83 μg·g-1 with variation coefficient 29.02%, the highest content resource was ‘Dalixiaoyuanzao’. In terms of the four components, the contents of maslinic acid were the highest, followed by betulinic acid, the contents of oleanolic acid and ursolic acid were lower. The genetic diversity of each component was abundant in different jujube resources, among which the variation coefficient of ursolic acid was the highest (60.75%). (2) The normal test results showed that the contents of maslinic acid, betulinic acid and total triterpenic acid were normally distributed, and the contents of oleanolic acid and ursolic acid were not normally distributed. (3) The correlation analysis results showed that the total triterpenic acid contents were significantly positive correlated with all four components, and there were also correlations between components, the maslinic acid contents were significantly positive correlated with betulinic acid, the betulinic acid content were significantly positive correlated with oleanolic acid, and the oleanolic acid content were significantly positive correlated with ursolic acid. (4) Cluster analysis can divide 219 resources into 4 groups, which were the groups with the highest content of ursolic acid, the groups with higher contents of oleanolic acid and ursolic acid, the groups with lower content of each components, and the groups with the higher contents of maslinic acid, betulinic acid and total triterpenic acid. (5) We screened out 13 germplasm resources including higher contents of each component and total triterpenic acid, which can provide raw materials for the development of triterpenic acid related functional products.

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XUE Xiaofang, ZHAO Ailing, WANG Yongkang, REN Haiyan, LI Dengke, LI Yi. Analysis of Triterpenic Acid Contents in Fruits of 219 Jujube Germplasm Resources[J]. Acta Botanica Boreali-Occidentalia Sinica,2021,41(3):480-492

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  • Online: April 23,2021
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