In order to study the function of the stelar K⁺ outward rectifier channels (SKOR) gene in Elytrigia elongata, we cloned EeSKOR promoter from E. elongata by thermal asymmetric staggered PCR (TailPCR) technology, and analyzed the cisacting elements and gene expression of the promoter. The results showed that: (1) the 798 bp promoter sequence of the EeSKOR gene was successfully obtained and named pEeSKOR. (2) This promoter also contains specific transcription factor binding sites, plant hormone response elements, light response elements, tissuespecific promoter elements and stress response elements in addition to the essential core promoter elements. (3) The plant expression vector pEeSKOR∷GUS was successfully constructed. Through Agrobacteriummediated transient transformation, the GUS reporter gene driven by the EeSKOR promoter can be expressed in the leaves, petioles and roots of Arabidopsis. (4) Realtime quantitative PCR detection showed that EeSKOR showed the different expression patterns in the roots of E. elongata, under NaCl, PEG, ABA and SA treatments. The expression of EeSKOR showed a trend of first downregulation and then upregulation under NaCl treatment, under PEG treatment, while the expression of EeSKOR gene was upregulated, and significantly upregulated with time. ABA treatment caused the expression of EeSKOR to be inhibited and decreased significantly with the extension of treatment time. The expression of EeSKOR showed a trend of upregulation and then downregulation under SA treatment, and the expression level of EeSKOR was significantly lower than the normal expression level after 72 h of SA treatment. Studies indicated that EeSKOR gene expression was regulated by NaCl, PEG, ABA and SA. The results of this study would provide an important theoretical basis for the further systematic study on the EeSKOR gene function in E. elongata.