WUSCHEL-related homeobox (WOX) transcription factors are plant-specific transcription factors that can regulate plant growth and development and the response to abiotic stress. In this study, the sequence of Glycyrrhiza uralensis WOX gene family was analyzed by bioinformatics, and the expression of this gene family under drought, salt, phosphorus free, abscisic acid (ABA) and gibberellin a3 (GA3) treatment were detected by real-time fluorescence quantitative PCR (RT-qPCR). The results showed that: (1) there were 16 members of the G. uralensis WOX family (GuWOX1-GuWOX16). The amino acids encoded by WOX genes were variable from 168 to 452 aa,with the protein molecular weight ranged from 19.39 to 50.11 KDa and The theoretical isoelectric point distribution ranged from 5.28 to 8.97. GuWOX contained conserved HD domains and were hydrophilic and unstable proteins. (2) Phylogenetic tree analysis showed that the WOX family was divided into 3 major clades, (e.g., the Ancient clade, the Intermediate clade and the Modern/WUS clade). (3) Numerous stress and hormone-responsive cis-elements were observed in the GuWOX promoter regions. (4)RT-qPCR analysis showed that most GuWOXs were induced under salt, phosphorus-free and GA treatments at different time periods. The expression patterns of GuWOXs under drought and ABA treatments were similar, and the expressions of GuWOXs were up-regulated after 12 h of treatment. This indicates that GuWOX transcription factor played a regulatory role in stress of G. uralensis. The results provided a foundation for further analysis of WOX gene family members in stress resistance of G. uralensis.