外源硫化氢和水杨酸对盐胁迫下加工番茄幼苗生长与生理特性的影响。
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石河子大学

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硫化氢信号系统调控加工番茄耐盐性


Effects of exogenous hydrogen sulfide and salicylic acid on growth and physiological characteristics of processing tomato seedlings under salt stress
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Hydrogen sulfide signaling system regulates salt tolerance of processed tomatoes

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    摘要:

    摘要:以加工番茄KT-7为材料,在水培条件下,研究外源水杨酸(SA,0.15mmol/L)、硫化氢(H2S)供体硫氢化钠(NaHS,50mmol/L)对150mmol/L NaCl胁迫下加工番茄幼苗的渗透调节、活性氧代谢和快速叶绿素荧光的影响,考察H2S和SA这2种信号分子协同作用、为探讨缓解加工番茄幼苗盐胁迫的生理机制提供理论依据。结果表明,外源SA、H2S处理及其复合处理均能有效缓解NaCl胁迫对加工番茄幼苗受到的NaCl胁迫伤害。使加工番茄幼苗叶绿素含量、叶片相对含水量、脯氨酸含量,较盐胁迫处理有不同程度的提高;而各外源SA、H2S处理的丙二醛(MDA)含量、电解质渗透率、过氧化氢(H2O2)含量分别较盐胁迫处理有不同程度的降低;其中根施外源0.15mmol/L SA和喷施50mmol/L H2S复配处理下加工番茄幼苗MDA含量、电解质渗透率及H2O2产生速率均达到最小,SA和H2S复配处理下加工番茄而复配处理幼苗的脯氨酸含量达到最大。综上所述研究发现,盐胁迫下外源SA和、H2S单一或复配处理均能提高加工番茄幼苗的耐盐性,且H2S和SA复配处理之间存在着一定程度的协同作用,可以诱导增强 NaCl 胁迫下加工番茄幼苗植株渗透调节能力的增加,进一步而提高加工番茄幼苗对盐胁迫的适应性。其结果可为探讨H2S和 SA这2 种信号分子协同作用、缓解加工番茄幼苗盐胁迫的生理机制提供理论依据。

    Abstract:

    Abstract: Using processed tomato KT-7 as material, under hydroponic conditions.The effects of exogenous salicylic acid (SA,0.15mmol/L) and hydrogen sulfide (H2S) donor sodium hydride (NaHS,50mmol/L) on osmotic regulation,reactive oxygen metabolism and rapid chlorophyll fluorescence of tomato seedlings under 150mmol/L NaCl stress were investigated,The synergistic effect of H2S and SA signaling molecules was investigated to provide theoretical basis for the physiological mechanism of alleviating salt stress in processed tomato seedlings. The results showed that exogenous SA,H2S and their combination treatments could effectively alleviate the NaCl stress injury of processed tomato seedlings. The chlorophyll content,relative water content of leaves and proline content of processed tomato seedlings were improved to different degrees compared with the salt stress treatment;However,the content of malondialdehyde (MDA),electrolyte permeability and hydrogen peroxide (H2O2) content of each exogenous SA and H2S treatment were decreased to different degrees compared with the salt stress treatment;Among them,the MDA content, electrolyte permeability and H2O2 production rate of the processed tomato seedlings under the combined treatment of root application of exogenous 0.15mmol/L SA and spraying of 50mmol/L H2S all reached the minimum,while the proline content of the mixed treatment seedlings reached the maximum.

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  • 收稿日期:2021-09-22
  • 最后修改日期:2022-01-18
  • 录用日期:2022-01-06
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