Q943
杨凌农业科技开发基金
在克隆了二棱大麦第３组ＬＥＡｃＤＮＡ，抗旱基因ＨＤＣＳ１的基因上，将其连接于ｐＢ１１２１的ＣａＭＶ３５Ｓ启动子和ＮＯＳ终止子之间，，构建了ＨＤＣＳ１的植物表达载体ｐＢＨＣ，并进行了ＰＣＲ和酶切鉴定，为进行植物抗旱基因工程研究创造了条件。
Based on the cloning of a drought tolerant gene HDCS1 ,which is one of the group 3 LEA cDNA,a plant expression vector pBHC was constructed for this gene.In pBHC the fragment of HDCS1 was ligated downstream to CaMV
郭卫东 郑学勤.抗旱基因HDCS1的植物表达载体构建[J].西北植物学报,1999,19(3):
