Comparison on the flower morphologic,pollen fertility alteration and utilization of male sterile line 160S in Brassica napus were conducted.The results showed that the temperature was the main factor to flower morphologic and pollen fertility alteration.Under male sterile conditions，the anthers of 160S were all smaller than that of normal ones and the pollens were completely male sterile.The percentage of pollen fertility,the self-pollenated pod-setting ratio and seed number per pod decreased along with temperature rising,and the fertility transformation direction was fertile,part fertile and then complete sterile.The results of test cross with varieties showed that all testers could restore the sterility of 160S at high restore ability.160S has high specific combining ability and heterosis,it can be used to produce hybrid seeds according to the two-line hybrid system model and is a prominent approach to utilizing heterosis in rapeseed.