With vermiculite culture,seed filling,and their antioxidant system and osmotic regulation substances of two vegetable soybean cultivars with different salt tolerance were studied under 100 mmol/L NaCl stress.The results showed that:(1)Seed enlargement was normal for salt resistant cultivar ‘Lülingtezao’,but seed enlargement was inhibited for salt susceptible cultivar ‘Lixianggaochan 95-1’ under NaCl stress for 15 h.(2)O^-·₂ producting rate,H₂O₂ and malondialdehyde (MDA) contents in seeds of two cultivars were significantly increased under NaCl stress,while the increasing amount of salt resistant cultivar ‘Lülingtezao’ was lower than those of salt susceptible cultivar ‘Lixianggaochan 95-1’.(3)During the period of NaCl stress,the activities of superoxide dismutase (SOD),ascorbate peroxidase (APX) and glutathione reductase (GR),the contents of ascorbic acid (AsA),soluble protein and soluble sugar of ‘Lülingtezao’ were relatively higher compared with those of ‘Lixianggaochan 95-1’,and its activities of peroxidase (POD) and catalase (CAT),content of reduced glutathione (GSH) maintained relatively higher values in the intermediate,later,early and intermediate periods,respectively,under NaCl stress compared with those of ‘Lixianggaochan 95-1’.These results indicated that ‘Lülingtezao’ could maintain higher and mutually concordant activities of SOD,POD and CAT,maintain efficient metabolism of AsA-GSH cycle and stronger osmotic regulation ability.These resulted in the effective inhibition of lipid peroxidation,which might be one of the important reasons for stronger salt tolerance of ‘Lülingtezao’.