Two floweringrelated MADS-box genes,named as AfAP3 and AfPI were cloned from flower bud of Aechmea fasciata through designing degenerate primer and RACE technology.The full cDNA fragment of AfAP3 was 957 bp and coded a polypeptide of 226 amino acids.The full cDNA fragment of AfPI was 808 bp and coded a polypeptide of 198 amino acids.Both genes had a typical MADS-box domain.RT-PCR analysis showed that these two genes were main expressed in floral organs;on the other hand,they were also expressed a little in root.After flower induction by exogenous ethylene,AfPI gene began to express in stem tip at 7 d,AfAP3 gene began to express later.