Hydroxycinnamoyl-coenzyme A:shikimate/quinate hydroxycinnamoyltransferase (HCT) is one of the key enzymes in plant chlorogenic acid synthesis.In this study,we isolated HCT gene by homologous cloning method from ‘Yali’,and named as PbHCT3.The full length of PbHCT3 cDNA is 1 731 bp,and the 1 317 bp open reading frame encodes 438 amino acids which containing two conservative sequences HHXXD and DFGWG and conservative structure MVVNVTVRES domain of acyltransferase.Realtime PCR analysis indicated that PbHCT3 showed a higher expression in peel,pulp and core of young fruit,young leaves,and alabastrum,but the expression in fruit decreased with the fruit growth.These results suggested the PbHCT3 is closely related to growth and development of younger tissue in ‘Yali’.