Enolase catalyze only one-step dehydration reaction in glycolytic pathway,and relate closely to plant stress resistance.Based on EST monoclones of enolase gene obtained from bromeliads full length cDNA library of ornamental bromeliads,its full length cDNA sequence was obtained by Primer Walking sequencing.The gene,named for GoEnolase1 (GenBank accession No.JN896863),consisted of 1 703 bp cDNA sequence,1 335 bp ORF(open reading frame) which encoded a protein with 445 amino acids residues,and a putative protein which was 47.9 kD at estimated molecular weight,5.7 at isoelectric point and had a ‘TIM-phosphate-binding superfamily’ conservative domain.The secondary structure of the protein was composed of alpha helix (44.49%),random coil (33.71%),extend strand (13.71%) and beta turn (8.09%) by SPOMA analysis.Furthermore,its tertiary structure was also build based on A chain of 2PSN.Through phylogenetic tree analysis,GoEnolase1 was gather to a same group with enolase protein in Oryza sativa,Zea mays and Elaeis guineensis.The research was beneficial to breeding for stress resistance in ornamental bromeliads.