Abstract:Through RT-PCR,two transcription factor genes,OsJAMYB (MYB transcription factor gene,Genbank no.AY026332) and OsERF3 (Ethylene Response Factor gene,Genbank no.AB036883) were isolated from rice ‘Xiushui 11’ after striped rice borer ［Chilo supperssalis (Walker)］ feeding and were confirmed after Blastn analysis.The length of OsJAMYB and OsERF3 fragments were 1 026 bp and 805 bp,respectively.By using molecular cloning techniques,specific expression vectors were gotten with specific directions and specific insert sequence including OsJAMYB and OsERF3 for transformation by Agrobacterium tumefaciens.After incubating,screening and differentiation with ‘Xiushui 11’ calli and A.tumefaciens,the sense and antisense transgenic plants including OsJAMYB and OsERF3 were gained.The PCR was used to amplify the genes in T0 transformed rice genomics DNA to confirm the inserts of OsJAMYB and OsERF3.The results will provide the knowledge of induced resistance and molecular mechanism of OsJAMYB and OsERF3 in rice.
