The endophytic fungi were isolated from root,stem and leaf of the host by tissue inoculation culture and five pathogenic fungi and four bacteria strains used as targeting microbes to test microbial inhibition activity by agar plate antagonistic action and modified agar gel diffusion methods.The results indicated that:(1)Eighteen fungal endophytic strains were isolated from the host.Most of them came from leaf,then root and stem,and the isolated strains attribute to four genera,two families,and two orders based on morphological characteristics.(2)For the isolated strains,ten of them were found to have some microbial inhibition activities against two or more pathogenic fungi,making up 55.6% of the total isolates，and eight isolated fungi strains showed inhibition activities against one or more bacteria,respectively,reaching up to 44.4% of the total isolates in number.The strain LBEFL001 and LBEFL006 had evident antifungal activities against 5 pathogenic fungal strains.They ascribed to Aspergillus sp.and Penicilium sp.individually.The strain LBEFR006 and LBEFS002 showed evident antibacterial activities to Staphylococcus aureus and Pseudomonas aeruginosa,respectively.They both ascribed to Fusidium sp..The strain LBEFL004 expressed evident antibacterial activities to Bacillus subtilis and S.aureus,belonging to Aspergillus sp..It was found that the medicinal plant Limonium bicolor had relatively abundant fungal endophytic resource,and the isolatded endophytic fungi had evident inhibition activities against plant pathogens and showed relatively broad spectrum,and meanwhile the isolates as a whole had a certain of selectivity for bacterial inhibition,and inclined to be more antagonistic to S.aureus in general.