In order to elucidate the evolutionary characteristics of Neotorularia humilia,a pair of universal primers for Ethylene Receptor 1 (ETR1) gene in Brassicaceae was chosen to amplify the homologous ETR1 in N.humilia by PCR,and 14 homologous sequences were cloned.Sequences were aligned by Bioedit and the similarities among these clones at nucleotide level were from 93％ to 98％,and the similarities among these protein amino acid sequences were 78%.Nucleotide diversity was measured by DnaSP v5,and 14 DNA haplotypes were obtained in 1 644～1 661 bp fragments of BhETR1 in N.humilia.Furthermore,phylogenetic analysis revealed that 14 clones were classed into three clades,one representative clone from each clade (cloning 2,cloning 29,and cloning 35) was chosen to blast with ETR1 genes from Arabidopsis thaliana (L.) Heynh.and Arabidopsis lyrata.The nucleotide similarities were both 78％.These results suggest that BhETR1 is a ETR1-like gene,and the high heterogeneity among 14 BhETR1 sequences might be caused by the complex genome of N.humilia.