In this study the expression analysis of PheTFL1 gene related to moso bamboo flowering provide the theory basis for the research of flowering mechanism of moso bamboo.PP2A gene was screened from nine candidate reference genes including UBC18,PP2A and EF1α,and so on,because of its stable expression in 11 kinds of different tissues and organs such as the leaves,immature inflorescence,inflorescence axis,branches and the cuticle of bamboo stem,and so on,which is used to correct the qRT-PCR results of PheTFL1 gene.The results showed that the low level expression of the PheTFL1 in leaves,branches and cuticle of bamboo stem of flowering bamboo was not significantly different from those of non-flowering bamboo,while there was high level expression in immature inflorescence and inflorescence axis.The expressions of PheTFL1 were detected at high level in leaves and roots of seedlings,but at lower expression in stems.Appreantly the PheTFL1 gene were abundantly expressed in young tissues which had meristematic ability,it may be involved in regulating the growth of the meristems,as well as in the development of flower.