Using HPLC technique,we studied the characteristics of the changes of the content of endogenous hormones (ZT,GA₃,IAA,ABA) in Lycium barbarum L..The results showed that:(1)The correlation between the seeds’ IAA contents and diameter and single fruit weight reached a significant positive correlation.The correlation between the seeds’ IAA contents and length reached a positive correlation.It indicated that IAA in seeds played an important role during the fruit development.(2)The content of GA₃ was the most in fruits and seeds.From the first rapid growth period (after flowering,2～8 d) to the slow growth phase (after flowering,8～25 d),the changes of GA₃ were similar to the changes of IAA in seeds;After the second period of rapid growth (after flowering,25～33 d),the content of GA₃ decreased rapidly.Otherwise,the content of IAA in seeds increased rapidly.The reason may be that GA₃ promoted the synthesis of IAA of seeds,and then IAA in seeds can help the elongation of the fruit cell.These results also showed that the endogenous hormone in seeds played an important role during the fruit development in L.barbarum L..There was a dynamic balance relationship among every kinds of hormones.The different hormones in fruits and seeds worked together to help the fruit development of L.barbarum L..