13 species belonging to 4 sections of the genus Lilium were crossed in 80 different cross combinations,their self- and cross-compatibility were studied.In order to overcome pre- and postfertilization barriers,2 different pollination methods and embryo rescue technique were used.The results indicated that self-compatibility of wild lilies was different,self-incompatibility of L.leichtlinii and L.davidi ‘unicdor cotton’ was investigated.Among the cross combinations within Sinomartagon section,only L.cernuum×L.pumilum,L.davidi ‘unicdor cotton’×L.cernuum and L.davidi×L.leichtlinii obtained hybrid embryos and seedlings with delaying pollination and embryo rescue technique.Cross compatibility between Sinomartagon and Lophophorum section was better than other cross combinations from different sections of the genus Lilium.Delaying pollination method was more effective than cut-style pollination for most of cross combinations.For the majority of selfed and crossed combinations,tissue culture could effectively prevent post-crossing barriers and obtained first filial generation seedlings.