28 endophytic fungi isolated from the root,stem,leaf and fruit of Berberis thunbergii cv.atropurpurea were cultivated in liquid medium and the cultures were dried,grinded,and then extracted with acetone.The antifungal activities of the acetone extracts to five species of botanical pathogenic fungi were tested.The strain with wider antimicrobial spectrum was identified by molecular biological technology.The antifungal activities of the strain’s mycelia and fermentation broth were determined respectively.The results showed that:(1)Five endophytic fungi (R₃,S₄,L₆,F₂ and F₆ ) has a wider antifungal spectrum in the tested endophytic fungi that isolated from B.thunbergii cv.atropurpurea.The S₄ strain that isolated from the stem exhibit the best antifungal activity,which provided more than 90% inhibition of mycelium growth rates in five test fungi species,93.06% inhibition of Fusarium solani.(2)S₄ was identified as Paraphaeosphaeria by molecular biological technology.(3)The inhibition of mycelium growth rates for F.solani was significantly positively related to the tested concentration (volume) of mycelium acetone extracts from S₄(r=0.99,y=15.334x+14.618).The inhibition rates for F.solani was significantly negatively related to the tested concentration (volume) of fermentation broth of S₄(r=0.99,y=-20.196x+64.984).In that way,fermentation broth of S₄ promotes the growth of F.solani at high concentration.The compound with antifungal activities of S₄ mostly consisted of mycelia.