In this study,the cDNA of a new MYB gene,designated as GhMYB113,was isolated from cotton leaf by in silico cloning and RT-PCR.The length of its ORF was 738 bp and encoded a protein with 246 amino acids.The genomic sequence of GhMYB113 was 1 927 bp in length and was composed of 3 exons and 2 introns.Predicted protein belonged to R2R3-MYB family and contained two MYB domains.Alignment of sequences showed that the deduced GhMYB113 protein was highly homologous to other MYB proteins from different species.Phylogenetic analysis indicated that GhMYB113 was closest to MYB of hybrid rose.The quantitative real-time PCR (qPCR） demonstrated that GhMYB113 highly expressed in root.After drought,salt and low-temperature stress treatment,qPCR is perfomed to detecting the expression pattern of GhMYB113 in cotton seedlings.It turned out that GhMYB113 responded to all the three kinds of abiotic stresses.It was speculated that GhMYB113 plays a important role in response to abiotic stresses including drought,salinity and low-temperature .