Glucosinolates are secondary metabolites found mainly in Cruciferae plants.The CYP79 family,belongs to cytochromes P450,had been shown to catalyze the conversion of amino acids to their corresponding oximes in the biosynthesis of cyanogenic glucosides.In the present study,one of the glucosinolates regulator genes,named as BnCYP79B1 (GenBank JX535391.1) was cloned by homologous cloning from three stains of Brassica napus.The cDNA sequence of its open reading frame (ORF) was as long as 1 625 bp,encoding a putative protein of 541 amino acid residues with a theoretical isoelectric point of 8.88.Sequence comparison with Brassica oleracea var.botrytis CYP79B1 showed that,BnCYP79B1 gene had 98.83% similarity of nucleotide sequence and 99.26% similarity of the amino acid sequence.Three different lines,w678 (high-stemed and high glucosinolates content in seed),w673 (high-stemed and low glucosinolates content in seed),and w578 (dwarf mutant with high glucosinolates content in seed),were estimated for BnCYP79B1 expression by real-time quantitative PCR analysis.The results showed that:BnCYP79B1 gene mainly expressed in roots of w678 and in leaves of w578,but with the highest expression of BnCYP79B1 in w678.In conclusion,wild-type line had higher BnCYP79B1 expression level than the dwarf mutant,and also the high glucosinolates content line had rather higher BnCYP79B1 expression level than the low glucosinolates content line.