1-MCP和CO2对‘南果梨’冷藏后货架期能量代谢特性的影响
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国家农业科技成果转化项目(2012GB2B000085);“十二五”科技支撑项目(2012BAD38B09);沈阳农业大学青年教师基金项目(20112010)


Effect of 1-MCP and CO2 Treatments on Energy Metabolism Characteristics of ‘Nanguo’ Pear Fruits during Shelf Life after Cold Storage
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    摘要:

    分别对‘南果梨’果实采用0.75 μL/L 1-甲基环丙烯(1-MCP)在20 ℃熏蒸20 h后装入保鲜袋(0.02 mm)、用2% CO2充气果实包装袋(0.04 mm)、以保鲜袋果实为对照,各处理组果实均置于(0±1) ℃贮藏5个月后移至室温下(18 ℃±3 ℃),测定各处理果实在货架期间的褐变度以及果实线粒体蛋白含量、MDA含量、ATP含量,以明确1-MCP和CO2对南果梨冷藏后果实货架期的能量代谢特性。结果显示:(1)1-MCP和CO2处理可不同程度延缓南果梨冷藏后货架期果实果心褐变指数和褐变度,且1-MCP处理效果更好,但CO2处理在货架后期反而使果实褐变度较对照提高。(2)1-MCP和CO2处理可有效抑制果实MDA含量增加,延缓细胞膜透性的升高,保持细胞完整性。(3)1-MCP处理有利于提高货架前期果实中线粒体蛋白质含量,能够在货架后期保持较高的ATP含量和能荷水平,而CO2处理在货架前期果实内含有较高水平的ATP,促进了果实内的能荷水平。研究发现,1-MCP和CO2处理均可以通过影响南果梨果实的能量代谢特性从而影响果实的成熟衰老进程,且1-MCP处理可以抑制货架前期的ATP含量和能量供应,有利于保持细胞膜完整性,抑制果心褐变的发生,延缓果实成熟衰老进程;而2% CO2处理与对照相比对果实能量代谢特性的影响不大。

    Abstract:

    The ‘Nanguo’ pears were packed with plastic bags (0.02 mm) after being fumigated for 20 h with 0.75 μL/L 1-MCP (1-Methylcyclopropene) or with plastic bags (0.04 mm) after filled with 2% CO2 and cold storage (0±1 ℃) for 5 months,then stored at ambient temperature(18 ℃±3 ℃) for investigation.The browning index,mitochondria protein content,MDA content and ATP content were determined in order to study the effect of 1-MCP and CO2 on pear fruits energy metabolism characteristics during the shelf life after cold storage.The results show that:(1)Both 1-MCP and CO2 treatments can delayed the core browning index and browning degree differently,and the 1-MCP was better than CO2;(2)Both 1-MCP and CO2 treatments helps to retarded the increase of MDA and membrane permeability,holds the integrity of membrane;(3)1-MCP treatment helps to increase the content of mitochondria protein during the prophase of shelf life.1-MCP treatment can also remain high ATP content and energy charge level in postphase of shelf life.And filled with CO2 treatment can keep a high level of ATP content and energy charge in prophase of shelf life.It is suggested that both 1-MCP and filled with CO2 can adjusted the postharvest senescence process through effect the energy metabolism characteristics.In this study,the effectiveness of 1-MCP treatment is better than filled with CO2 on inhibited the core browning and kept the integrity of membrane.

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程顺昌,魏宝东,朱益鹏,等.1-MCP和CO2对‘南果梨’冷藏后货架期能量代谢特性的影响[J].西北植物学报,2013,33(6):1177-1182

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  • 在线发布日期: 2013-07-07
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