In the present study,a total of 327 F₂ lines from the cross between two common wheat Chinese Spring(female)×Lankaodali were planted in Qianxian,Qishan and Yangling of Shaanxi Province,respectively.Test weight,hardness,protein content and bound moisture content were evaluated.A genetic map was constructed based on 167 SSRs and 2 EST markers.QTL analysis was conducted with single environment model and multi-environment model by the software QTL-ICIMapping V.3.2.The results showed that:(1)In two mapping models,6 QTLs(QTW-6B-6,QTW-7B-6,QTW-7B-9,QTW-5D-2,QTW-6D-1,QTW-6D-4) were found for test weight,explaining 1.99%~6.57% of phenotypic variance in single-environment model,whereas accounting for 3.66%~20.07% of phenotypic variance in multi-environment model.And Q_MTW-7B-9,Q_MTW-6D-1 and Q_MTW-6D-4 showed major QTL in multi-environment model;(2)3 QTLs (QHD-4A-5,QHD-7A-1 and QHD-7B-9) were detected for hardness,and in single-environment model it explain phenotypic variance from 6.00% to 6.95%,while in multi-environment model it explain phenotypic variance account for 5.43%~9.64%;(3)1 QTL（QPR-6D-1） for protein content was mapping on 6D chromosome,explaining 5.39% and 10.06% of phenotypic variance in single-environment model and multi-environment model,respectively;(4)1 QTL for moisture content was detected explaining 39.20% and 75.01% of phenotypic variance in single environment model and multi environment model,respectively;(5)The QTLs controlling test weight,hardness,protein content and bound moisture content were mapping on 1B chromosome,indicating the big influences of 1B on these traits.Test weight,seed hardness,protein content and moisture content are mainly controlled by additive effects.The identified molecular markers related to these quality traits in this study will benefit for marker-assisted selection in breeding programs.