Temperature and light conditions were explored to find out the optimum conditions of spore germination of Polytrichum commune and Haplocladium microphyllum.To find the suitable method for rapid detect of spore viability of mosses,I²-KI reaction method,TTC reaction method,red ink staining method,and methylene blue staining method were studied based on six species of mosses by comparing the spore viability with the germination results.The results indicated that methylene blue staining method got the most similar results with the germination rate of moss spores and showed clear staining effect,which can be used for the rapid detection of the viability of bryophyte spores.The correlation between spore viability (x) detected by methylene blue staining and germination rate (y) in vitro reaches a very significant level（r=0.976）.The regression equation between spore germination ratio (y) and viability (x) can be obtained:y=-8.547+1.069x(P<0.01).The germination rate can be detected conveniently by spore viability.