The effects of medium components and culture conditions on pollen germination and pollen tube growth of Calanthe tsoongiana T.Tang et F.T.Wang in Tianmu Mountain were studied by in vitro culture,which was also used to investigate the pollen storage capacity.The stigma receptivity was estimated by benzidine-H₂O₂ method.The aim of present study was to screen the ideal culture methods and storage conditions for C.tsoongiana pollens,which would lay the foundation for the research of its germplasm resources preservation.The results showed that:(1)The optimum culture medium for pollen germination consisted of 200 g·L^-1 sucrose,50 mg·L^-1 H₃BO₃ and 40 mg·L^-1 Ca(NO₃)₂·4H₂O,in which the pollen germination percentage was nearly 81.71% and the average pollen tube length reached 247.42 μm after 48 h incubation under the optimum culture conditions involved pH 5.5～6.0 and 25 ℃.(2)Germination rate of dried pollens still maintained at 48.58% after 360 d storage under -80 ℃.(3)The stigma receptivity of C.tsoongiana could last 5 d after blooming,but the pollinarium kept a certain viability (28.96%~81.71% germination rate) during whole florescence.However,both stigma receptivity and pollen viability declined significantly with the extension of time after blooming.