硝酸镧浸种对NaCl胁迫下柳枝稷种子萌发及幼苗生理特性的影响
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国家“十二五”科技支撑计划(2011BAD17B05);中央高校基本科研业务费(Z109021205);西北农林科技大学博士科研启动基金(Z109021111)[ZK)]


Effect of Lanthanum Nitrate on Seed Germination and Seedling Growth Characteristics of Switchgrass under NaCl Stress
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    摘要:

    为探讨镧对NaCl胁迫下柳枝稷种子萌发及幼苗生理特性的影响,该研究以柳枝稷(Panicum virgatum L.)种子为研究材料,通过不同浓度(0、0.05、0.1、0.5和1.0 mmol/L)La(NO3)3溶液浸种24 h处理后,添加100 mmol/L NaCl溶液,以蒸馏水为对照,在种子发芽箱中培养并观察柳枝稷种子萌发和幼苗生长,测定幼苗叶片相对电导率、丙二醛、脯氨酸和叶绿素的含量。结果显示:(1) 0.05 mmol/L La(NO3)3 浸种处理可缓解100 mmol/L NaCl对种子发芽的影响。(2)不同处理对幼苗苗长无显著影响,0.1 mmol/L La(NO3)3浸种显著增加了幼苗的鲜重和叶面积,当浓度≥0.5 mmol/L时根长、根冠比、鲜重和叶面积受到抑制。(3) 幼苗叶片相对电导率和脯氨酸含量随La(NO3)3浓度的增加呈先降低后增加的趋势,其中0.05和0.1 mmol/L La(NO3)3浸种处理下效果较好,丙二醛含量随La(NO3)3浓度的增加持续降低,但各浓度处理间差异不显著,叶绿素含量在0.05 mmol/L La(NO3)3浸种处理下较NaCl处理显著增加,当浓度≥0.5 mmol/L随着浸种浓度的增加呈下降趋势。(4)不同指标间发芽势与发芽率、根长和叶绿素含量间呈极显著正相关关系,与根冠比、电导率和脯氨酸含量均呈极显著负相关关系,而与苗长不相关。研究表明,低浓度(0.05 mmol/L)La(NO3)3可缓解NaCl胁迫对柳枝稷种子萌发及幼苗生长的影响,而高浓度(1.0 mmol/L)的La(NO3)3则会加重NaCl的胁迫危害。

    Abstract:

    To investigate the effect of La(NO3)3 on seed germination and seedling growth characteristics of switchgrass(Panicum virgatum L.) under NaCl stress,we measured seed germination,seedling growth,relative electrical conductivity,MDA,proline and chlorophyll contents in incubator.Seeds were soaked in different concentrations (0,0.05,0.1,0.5 and 1.0 mmol/L) of La(NO3)3 for 24 h,and then added 100 mmol/L NaCl solution,with distilled water as control.The results showed that:(1)0.05 mmol/L La(NO3)3 could relieve 100 mmol/L NaCl effect on seed germination;(2)There was no significant effect on shoot length with different treatments,but 0.1 mmol/L La(NO3)3 treatment significantly increased the fresh weight and leaf area of seedlings.When the concentration≥0.5 mmol/L,root length,root shoot ratio,fresh weight and leaf area were all inhibited significantly;(3)The relative electrical conductivity and proline content of seedling leaves decreased firstly and then increased with increasing La(NO3)3 concentration,of which 0.05 and 0.1 mmol/L La(NO3)3 treatment were better,the MDA content increased with increasing La(NO3)3 concentration has been reduced.The chlorophyll content increased with 0.05 mmol/L La(NO3)3 treatment comparing with NaCl,and decreased with increasing La(NO3)3 concentration.However,there were no significant difference among different concentration treatments.(4)Different factors between the germination potential and germination rate,had a significantly positive correlation between root length and chlorophyll content,and had a significantly negative correlation with the root shoot ratio,relative electrical conductivity,and proline content.However,it was no significant correlation between germination potential and shoot length.This research indicated that low concentration (0.05 mmol/L) of La(NO3)3 could alleviate NaCl stress,high concentration (1.0 mmol/L) of La(NO3)3 could increase the stress.

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何学青,梁卫卫,常乐钦,等.硝酸镧浸种对NaCl胁迫下柳枝稷种子萌发及幼苗生理特性的影响[J].西北植物学报,2014,34(3):543-549

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  • 在线发布日期: 2014-04-10
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