复硝酚钠对韭菜生长及硝酸盐还原同化效应研究
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河北省科技支撑计划项目(11220701D);河北省自然科学基金(C2013204076)


Effects of Compound Sodium Nitrophenolate on Growth and Nitrate Reduction and Assimilation in the Leaves of Chinese Chive
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    摘要:

    于收割后第12天对日光温室韭菜分别叶面喷施0.15、0.25、0.50 mL·L-1复硝酚钠(CSN),并测定分析CSN处理不同时间韭菜生物量、叶片NO3-累积、氮代谢关键酶以及相关代谢产物含量的变化,探讨CSN对韭菜硝酸盐累积污染的减控效应及其相关生理机制。结果显示:(1)韭菜硝酸盐含量在叶面喷施CSN后呈先降低(3~6 d)后增加(6~9 d)的趋势,并以0.15 mL·L-1 CSN处理至第6天的韭菜硝酸盐含量最低,比对照降低了29.6%。(2)不同浓度CSN处理可显著提高韭菜叶片氮代谢关键酶的活性,其中0.15 mL·L-1 CSN处理后第6天叶片硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、谷氨酸草酰乙酸转氨酶(GOT)和谷氨酸丙酮酸转氨酶(GPT)活性分别较对照增加了34.4%、61.3%、208.8%、7.4%。(3)各浓度CSN处理可明显促进韭菜生长,提高韭菜营养品质,0.15 mL·L-1 CSN处理后第6天叶片干、鲜重分别较对照增加10.7%、10.8%,叶片游离氨基酸含量、可溶性蛋白质含量、Vc含量分别较对照提高23.1%、23.3%、0.5%。研究表明,在韭菜氮代谢的还原同化途径中,CSN处理不仅能够显著提高氮还原动力泵(NR)和氮同化初级动力泵(GS)活性,而且能够同时调动氮同化次级动力泵(GOT和GPT)的转氨作用积极协同配合,还可能调动碳同化产物的积极协同配合(即可溶性糖含量降低),来促进硝态氮转化为游离氨基酸和可溶性蛋白以及次生代谢物质Vc的合成,减少了硝酸盐进入液泡贮积。

    Abstract:

    In a greenhouse experiment,different doses (0.15,0.25,0.50 mL·L-1) of compound sodium nitrophenolate (CSN) were used to spray the Chinese chives cultivar ‘Dajingou’ on the 12th day of post-harvest.The effects of CSN on yield,nitrate metabolism and quality was investigated after different treatment days (0,3,6,9,12 d).The results showed that:(1)The time dynamic patterns of nitrate concentrations had simple valley values,which appeared on 6 d,and the reduction by 29.6% occurred for 0.15 mL·L-1 CSN in comparison with control.(2)This significant decrease was accompanied with elevated activity of nitrate reductase(NR),glufaminc synthctasc(GS),glutamate pyruvate transaminase(GOT) and glutamate oxaloacetate transaminase(GPT) by 34.4%,61.3%,208.8% and 7.4%,respectively.(3)The increase in yield by 10.7% for dry matter and 10.8% for fresh matter accompanied with an increase in free amino acid by 23.1%,soluble protein by 23.3%,and Vc by 0.5% in CSN treatment compared to control.According to the results obtained,CSN obviously enhanced the activities of NR(which was named as ‘driving-pump of nitrate reduction’) and GS(which was called ‘first-driving-pump of nitrogen assimilation’),and might also promoted contribution of free amino acid,dissolvable protein and Vc by transamination action of GOT and GPT(which were called ‘second-driving-pump of nitrogen assimilation’) to reduce nitrate accumulation in vacuole of Chinese chives cells.

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李红利,王俊玲,薛占军,等.复硝酚钠对韭菜生长及硝酸盐还原同化效应研究[J].西北植物学报,2014,34(4):740-745

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  • 在线发布日期: 2014-04-29
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