Expression pattern of GmARF10 gene was analyzed by real-time quantitative PCR(qPCR) method.The working model between Gm-miR160 and its targets was investigated by mining soybean and Arabidopsis genome data,and the resistant version vector GmARF10∷mGmARF10(mGmARF10) was constructed.The mGmARF10 vector was transformed into wild type soybean by Agrobactium-mediated method and transgenic plants were phenotyped physiologically and molecularly.The results showed that transcripts of GmARF10 exhibit the highest levels in floral organs and the lowest levels in stems among the tested soybean organs.Moreover,its transcriptional level was found lower in first trifoliolate leaves than either in cotyledons or in unifoliolate leaves.There is no dramatically difference between the transgenic leaves and the control leaves in respect of leaf shape and size.However,the leaves of transgenic plants show decreased levels in chlorophyll content and maximum quantum efficiency(F_v/F_m) and increased transcription of GmCYSP1,a senescence-associated marker.Taken together,these results suggest that the soybean auxin response factor GmARF10 may be involved in leaf senescence and likely act as a positive regulator in leaf senescence.