蒺藜苜蓿叶片光合作用对盐胁迫的响应
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国家自然科学基金(31100196);高等学校博士学科点专项科研基金(20110062120015)


Photosynthesis in Leaves of Medicago truncatula under Salt Stress
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    摘要:

    为阐明蒺藜苜蓿(Medicago truncatula)叶片光合效率对盐胁迫的响应规律,明确其土壤盐分阈值,该研究以盆栽蒺藜苜蓿幼苗为研究对象,采用加盐的方式人工模拟盐胁迫环境,设置不同浓度NaCl处理(0、50、100、150、200、250、300、400 mmol·L-1),利用Li-6400便携式光合测定仪分析了蒺藜苜蓿幼苗光合效率参数对土壤盐分浓度的响应特征。结果表明:(1)蒺藜苜蓿叶片净光合速率(Pn)和光合作用特征参数等具有明显的土壤盐分临界效应。在NaCl浓度为100~200 mmol·L-1时,蒺藜苜蓿可维持较高光合生产力,此盐分范围内适宜的光合有效辐射(PAR)为600~1 300 μmol·m-2·s-1,出现Pn最大值(20.7 μmol·m-2·s-1)的NaCl浓度为150 mmol·L-1,对应PAR为1 200 μmol·m-2·s-1左右。(2)在NaCl浓度<150 mmol·L-1时,随着NaCl浓度的增加,表观量子效率(AQY)、光补偿点(LCP)、暗呼吸速率(Rd)和最大光合速率(Pnmax)逐渐增大;在NaCl浓度为150 mmol·L-1时,AQYRdPnmax分别达到最大值0.030、0.605 7 μmol·m-2·s-1、19.4 μmol·m-2·s-1,而LCP达到最小值19.8 μmol·m-2·s-1。(3)NaCl浓度为150 mmol·L-1可作为导致蒺藜苜蓿净光合速率下降的气孔限制和非气孔限制因素的转折点,并且随着NaCl浓度升高,其光合速率由气孔限制转为非气孔限制的PAR降低。以上结果表明,蒺藜苜蓿对盐胁迫具有较强的适应性,在较高盐分浓度下可获得较高的光合生产力。

    Abstract:

    To investigate the photosynthetic efficiency in leaves of Medicago truncatula and its threshold in response to the salt stress in the soil,we used Li-6400 photosynthesis system to measure the photosynthetic parameters of the pot-grown seedlings under different concentrations of salt treatments(0,50,100,150,200,250,300 and 400 mmol·L-1).The results showed that:(1)The net photosynthetic rate(Pn) and characteristic parameters of photosynthesis in leaves of M.truncatula displayed threshold-value in response to variations in salt concentration.The M.truncatula plants were able to maintain higher photosynthetic productivity and the range of the suitable photosynthetic active radiation(PAR) was 600~1 300 μmol·m-2·s-1 at NaCl concentration of 100~200 mmol·L-1,the maximum of Pn(20.7 μmol·m-2·s-1) appeared at NaCl concentration of 150 μmol·mol-1,and the corresponding PAR was 1 200 μmol·m-2·s-1.(2)When the NaCl concentration was lower than 150 mmol·L-1,the apparent quantum yield(AQY),light compensation point(LCP),respiration rate(Rd) and maximum photosynthesis rate(Pnmax) increased with the NaCl concentration increasing.The maximum AQY(0.030),Rd(0.605 7 μmol·m-2·s-1) and Pnmax(19.4 μmol·m-2·s-1) as well as the minimum LCP(19.8 μmol·m-2·s-1) appeared when treated with 150 mmol·L-1 NaCl.(3)According to the theory of stomatal limitation,it is the turning point of 150 mmol·L-1 NaCl,which led to the depression of Pn from stomatal limitation to non-stomatal limitation and the PAR from stomatal limitation to non-stomatal limitation reduced with increasing NaCl concentration.To sum up,M.truncatula had high adaptability to salt stress,and had high photosynthetic productivity in high salt concentration.

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李紫薇,马天意,梁国婷,等.蒺藜苜蓿叶片光合作用对盐胁迫的响应[J].西北植物学报,2014,34(10):2070-2077

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  • 在线发布日期: 2014-10-31
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